Exp Mol Med.
2001 Dec;33(4):205-208.
Study of ethidium bromide interaction peculiarities with DNA
- Affiliations
-
- 1Biophysics Department of the Biological Faculty of Yerevan State University, Armenia. kensafiz@ysu.am
Abstract
- The helix-coil transition of DNA-ethidium bromide complexes in an interval of ionic strength of 2.0 x 10(-3) M < or = muNa+ < or = 2.0 x 10(-2) M has been investigated. It has been revealed that at the certain high ligand-DNA ratios (r(b)) the transition interval of the complex--(deltaT) becomes equal to that of DNA itself (deltaTo). It has been shown that the values of r(b) at which delta deltaT=deltaT-deltaT0=0 depends on ionic strength of a solution. Further increasing of ligand concentration leads to its conversion from stabilizer into the destabilizer of the double-stranded DNA.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Cooperative binding interaction of ethidium with allosteric DNA
- The binding of ethidium bromide with DNA: interaction with single- and double-stranded structures
- Effects of Antioxidants on Ethidium Bromide-induced Inhibition of Insulin Secretion in Rat Pancreatic Islets
- Apoptotic process is involved in the L-glutamate-induced PC12 cell death
- The Optimal Conditions of Chromosomal Analysis in Peripheral Blood
