Exp Mol Med.
2003 Dec;35(6):527-533.
The binding of ethidium bromide with DNA: interaction with single- and double-stranded structures
- Affiliations
-
- 1Biophysics Department of the Biological, Faculty of Yerevan State University, 375025 Yerevan, Aleck Manoogian St. 1, Armenia. simvar@arminco.com
- 2Physics Department, Yerevan University of Architecture and Construction, 375009, Yerevan, Teryan St. 105, bld.2.
Abstract
- The pH-induced helix-coil transition of DNA and its complexes with EtBr is carried out at acidic pH in a wide interval of change of concentration ratio of EtBr/DNA. The binding isotherms of EtBr on double and single-stranded DNA at pH = 7.0 and pH = 3.0 (t = 25 degrees C) are obtained by absorption and fluorimetric methods. Binding constants (K) and number of bases (n), corresponding to one binding site were determined. Non fluorescent "strong" complex with ds-DNA at pH = 7.0 and t = 25 degrees C as well as "strong" and "weak" complexes with ss-DNA at pH = 3.0 and t = 25 degrees C are revealed.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Study of ethidium bromide interaction peculiarities with DNA
- Cooperative binding interaction of ethidium with allosteric DNA
- Nuclear Factors Bind to the Specific Sites in Promoter Region of Human YB-1 Gene
- Environments of ethidium binding to allosteric Dna: II. Accessibility, mobility and mode of binding
- DNA damage to human genetic disorders with neurodevelopmental defects
