Int J Oral Biol.  2010 Sep;35(3):91-97.

Effects of Chitosan on the Differentiation of MDPC-23 Cells

Affiliations
  • 1Oral Biology Research Institute, Chosun University School of Dentistry, Gwangju, Korea. hjbkim@chosun.ac.kr
  • 2Division in Anatomy and Developmental Biology, Department of Oral Biology, Brain Korea 21 Project, Yonsei University College of Dentistry, Seoul, Korea.

Abstract

The effects of chitosan upon the experimentally induced differentiation of MDPC-23 cells, derived from mouse dental papilla cells, were investigated by RT-PCR, observations of cell morphology and Alizaline red-S staining. Chitosan was found to significantly increase and accelerate the expression of ALP mRNA but decrease the ColI transcript levels, as compared with the control, in a time-dependent manner during the differentiation of MDPC-23 cells. Chitosan also significantly downregulated ON mRNA expression and accelerated mineralization in differentiating MDPC-23 cells. These results suggest that chitosan facilitates odontoblast differentiation and mineralization and may have potential clinical applications as a dentin regeneration material.

Keyword

chitosan; odontoblast; differentiation

MeSH Terms

Animals
Chitosan
Dental Papilla
Dentin
Mice
Odontoblasts
Regeneration
RNA, Messenger
Chitosan
RNA, Messenger
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