Korean J Vet Res.  2011 Jun;51(2):139-149.

Overexpression of Galectin-3 in Macrophages of C57BL/6 mice with Experimental Autoimmune Encephalomyelitis

• Kim DS
• Hwang I
• Park SJ
• Ahn G
• Park SJ
• Park HJ
• Joo HG
• Jee Y

Abstract

Experimental autoimmune encephalomyelitis (EAE) is an inflammatory disease in the murine central nervous system (CNS) and has long been used as an animal model for human multiple sclerosis. Development of EAE requires coordinated expression of a number of genes that are involved in the activation and effector functions of inflammatory cells. Galectin-3 (Gal-3) is a member of the beta-galactoside-binding lectin family and plays an important role in inflammatory responses through its functions on cell activation, cell migration or inhibition of apoptosis. We investigated the functional role of Gal-3 in EAE mice following immunization with myelin oligodendrocyte glycoprotein (MOG)35-55 peptide. During the peak stage of EAE, the localization of Gal-3 in inflammatory cells markedly increased in subarachnoid membranes and perivascular regions of CNS. In contrast, Gal-3 was weakly detected in cerebrum and spinal of the recovery stage of EAE. Consistent with this finding, western blot analysis revealed that Gal-3 expression was significantly increased at the peak stage while it was slightly decreased at the recovery stage in the CNS. In addition, the population of CD11b+ macrophage expressing Gal-3 in spleen of EAE mice was markedly increased compared with control mice. In fact, most of activated macrophages isolated from spleen of EAE mice expressed Gal-3. Taken together, our results demonstrate that the over-expression of Gal-3 in activated macrophages may play a key role in promoting inflammatory cells in the CNS during EAE.

Keyword

Central Nervous System; Experimental Autoimmune Encephalomyelitis; Galectin-3; macrophage

MeSH Terms

Animals
Apoptosis
Blotting, Western
Cell Movement
Central Nervous System
Cerebrum
Encephalomyelitis, Autoimmune, Experimental
Galectin 3
Humans
Immunization
Macrophages
Membranes
Mice
Models, Animal
Multiple Sclerosis
Myelin-Oligodendrocyte Glycoprotein
Spleen
Galectin 3
Myelin-Oligodendrocyte Glycoprotein