Korean J Pathol.  2011 Jun;45(3):271-275. 10.4132/KoreanJPathol.2011.45.3.271.

Copy Number Alterations of BCAS1 in Squamous Cell Carcinomas

Affiliations
  • 1Department of Pathology, The Catholic University of Korea College of Medicine, Seoul, Korea. suyoung.dr@gmail.com
  • 2Department of Hospital Pathology, The Catholic University of Korea College of Medicine, Seoul, Korea.

Abstract

BACKGROUND
Breast carcinoma amplified sequence 1 (BCAS1), located in 20q13, is amplified and overexpressed in breast cancers. Even though BCAS1 is expected to be an oncogene candidate, its contribution to tumorigenesis and copy number status in other malignancies is not reported. To elucidate the role of BCAS1 in squamous cell carcinomas, we investigated the copy number status and expression level of BCAS1 in several squamous cell carcinoma cell lines, normal keratinocytes and primary tumors.
METHODS
We quantitated BCAS1 gene by real-time polymerase chain reaction (PCR). Expression level of BCAS1 was measured by real-time reverse transcription-PCR and immunoblot.
RESULTS
Seven (88%) of 8 squamous cell carcinoma cell lines showed copy number gain of BCAS1 with various degrees. BCAS1 gene in primary tumors (73%) also showed copy number gain. However, expression level did not show a linear correlation with copy number changes.
CONCLUSIONS
We identified copy number gain of BCAS1 in squamous cell carcinomas. Due to lack of linear correlation between copy numbers of BCAS1 and its expression level, we could not confirm that the overexpression of BCAS1 is a common finding in squamous cell carcinoma cell lines. However, this study shows that the copy number gain of BCAS1 is a common finding in squamous cell carcinomas.

Keyword

Gene dosage; DNA copy number variations; BCAS1 protein, human; Gene expression; Carcinoma, squamous cell

MeSH Terms

Breast
Carcinoma, Squamous Cell
Cell Line
Cell Transformation, Neoplastic
Coat Protein Complex I
DNA Copy Number Variations
Gene Dosage
Gene Expression
Keratinocytes
Neoplasm Proteins
Oncogenes
Real-Time Polymerase Chain Reaction
Coat Protein Complex I
Neoplasm Proteins
Full Text Links
  • KJP
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr