J Korean Soc Pediatr Nephrol.  2011 Apr;15(1):29-37. 10.3339/jkspn.2011.15.1.29.

Pathology of Glomerulonephritis: Specimen Preparation and Basic Pathologic Changes

• Kim YJ

• KMID: 1442143
• DOI: http://doi.org/10.3339/jkspn.2011.15.1.29

Abstract

To understand the course of renal diseases well, we must have basic knowledges of histologic procedures of renal biopsy samples as well as basic pathologic changes. This article describes the method of dividing the biopsy samples , fixatives for various pathologic examinations and basic pathologic changes of glomerular diseases. For light microscopic examination, color changes of glomerular structures in PAS, trichrome and PAM stains, normal glomerular patterns compared to various glomerulopathies are introduced. While describing typical staining patterns and intensities of fluorescence in membranous glomerulopathy and IgA nephropathy, basic interpretation of immunofluorescent microscopic examination is described. To understand electron microscopic pictures of renal diseases, preference locations of electron dense deposits in various glomerulonephrites are described with schema. This article is the introduction part of the renal pathology and for the further detail changes of specific entities, we should reference the renal pathology textbooks or articles.

Keyword

Glomerulonephritis; Pathology; Renal biopsy; Light microscopy; Immunofluorescence microscopy; Immunohistochemistry; Electron microscopy

MeSH Terms

Biopsy
Coloring Agents
Electrons
Fixatives
Fluorescence
Glomerulonephritis
Glomerulonephritis, IGA
Glomerulonephritis, Membranous
Immunohistochemistry
Light
Microscopy, Electron
Microscopy, Fluorescence
Coloring Agents
Fixatives