Lab Anim Res.  2012 Jun;28(2):83-90. 10.5625/lar.2012.28.2.83.

Effects of male silkworm pupa powder on the erectile dysfunction by chronic ethanol consumption in rats

• Oh HG
• Lee HY
• Kim JH
• Kang YR
• Moon DI
• Seo MY
• Back HI
• Kim SY
• Oh MR
• Park SH
• Kim MG
• Jeon JY
• Shin SJ
• Ryu KS
• Chae SW
• Kim O
• Park JK

• KMID: 1436699
• DOI: http://doi.org/10.5625/lar.2012.28.2.83

Abstract

Erectile dysfunction (ED) is a highly prevalent disorder that affects millions of men worldwide. ED is now considered an early manifestation of atherosclerosis, and consequently, a precursor of systemic vascular disease. This study was designed to investigate the effects of male silkworm pupa powder (SWP) on the levels of nitric oxide synthase (NOS) expression, nitrite, and glutathione (GSH); lipid peroxidation; libido; and erectile response of the corpus cavernosum of the rat penis. We induced ED in the study animals by oral administration of 20% ethanol over 8 weeks. The SWP-treated male rats were divided into 3 groups that were orally administered 200, 400, and 800 mg/kg. The libido of the SWP-administered male rats was higher than that of the ethanol control group. In addition, the erectile response of the corpus cavernosum was restored in males on SWP administration, to a level similar to that of the normal group without ED. The testosterone concentration did not increase significantly. The lipid peroxidation in the corpus cavernosum of the male rats administered SWP decreased significantly. In contrast, compared to the ethanol group, SWP-administered male rats showed increased GSH levels in the corpus cavernosum. The level of nitrite and NOS expression in the corpus cavernosum of SWP-administered male rats increased significantly. These results indicated that SWP effectively restored ethanol-induced ED in male rats.

Keyword

Erectile dysfunction; libido activity; sexual function; silkworm pupa powder

MeSH Terms

Administration, Oral
Animals
Atherosclerosis
Bombyx
Erectile Dysfunction
Ethanol
Glutathione
Humans
Libido
Lipid Peroxidation
Male
Nitric Oxide Synthase
Penis
Pupa
Rats
Testosterone
Vascular Diseases
Ethanol
Glutathione
Nitric Oxide Synthase
Testosterone

Figure

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  Figure 1 Effect of the silkworm pupa powder (SWP) on the erectile response to carvernous nerve stimulation in chronic ethanol-treated rats. a,bValues in the row with different superscripts are significantly different, P<0.05. Data are shown as the mean±SD (n=8).

• 

  Figure 2 Effect of the silkworm pupa powder (SWP) on the blood testosterone and protein level in chronic ethanol-treated rats. Data are shown as the mean±SD (n=8).

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  Figure 3 Effect of the silkworm pupa powder (SWP) on the lipid peroxidation level in corpus cavernosum of chronic ethanol-treated rats. a,bValues in the row with different superscripts are significantly different, P<0.05. Data are shown as the mean±SD (n=8).

• 

  Figure 4 Effect of the silkworm pupa powder (SWP) on the glutathione level in corpus cavernosum of chronic ethanol-treated rats. a,b,cValues in the row with different superscripts are significantly different, P<0.05. Data are shown as mean±SD (n=8).

• 

  Figure 5 Effect of the silkworm pupa powder (SWP) on the nitrite level in corpus cavernosum of chronic ethanol-treated rats. a,b,cValues in the row with different superscripts are significantly different, P<0.05. Data are shown as the mean±SD (n=8).

• 

  Figure 6 Effect of the silkworm pupa powder (SWP) on the nitric oxide synthase (NOS) in corpus cavernosum of chronic ethanol-treated rats. a,bValues in the row with different superscripts are significantly different, P<0.05. Data are shown as the mean±SD (n=8).

Reference

1. Maas R, Schwedhelm E, Albsmeier J, Boger RH. The pathophysiology of erectile dysfunction related to endothelial dysfunction and mediators of vascular function. Vasc Med. 2002. 7(3):213–225.

2. Benet AE, Melman A. The epidemiology of erectile dysfunction. Urol Clin North Am. 1995. 22(4):699–709.

3. Cheng JY, Ng EM, Chen RY, Ko JS. Alcohol consumption and erectile dysfunction: meta-analysis of population-based studies. Int J Impot Res. 2007. 19(4):343–352.

4. Christ GJ. The penis as a vascular organ. The importance of corporal smooth muscle tone in the control of erection. Urol Clin North Am. 1995. 22(4):727–745.

5. Hedlund H, Andersson KE. Contraction and relaxation induced by some prostanoids in isolated human penile erectile tissue and cavernous artery. J Urol. 1985. 134(6):1245–1250.

6. Saenz de Tejada I, Blanco R, Goldstein I, Azadzoi K, de las Morenas A, Krane RJ, Cohen RA. Cholinergic neurotransmission in human corpus cavernosum. I. Responses of isolated tissue. Am J Physiol. 1988. 254(3):H459–H467.

7. Saenz de Tejada I, Goldstein I, Azadzoi K, Krane RJ, Cohen RA. Impaired neurogenic and endothelium-mediated relaxation of penile smooth muscle from diabetic men with impotence. N Engl J Med. 1989. 320(16):1025–1030.

8. Furchgott RF. Introduction to EDRF research. J Cardiovasc Pharmacol. 1993. 22(Suppl 7):S1–S2.

9. Ignarro LJ, Buga GM, Wood KS, Byrns RE, Chaudhuri G. Endothelium-derived relaxing factor produced and released from artery and vein is nitric oxide. Proc Natl Acad Sci USA. 1987. 84(24):9265–9269.

10. Forstermann U, Munzel T. Endothelial nitric oxide synthase in vascular disease: from marvel to menace. Circulation. 2006. 113(13):1708–1714.

11. Garg UC, Hassid A. Nitric oxide-generating vasodilators and 8-bromo-cyclic guanosine monophosphate inhibit mitogenesis and proliferation of cultured rat vascular smooth muscle cells. J Clin Invest. 1989. 83(5):1774–1777.

12. Ross R. Atherosclerosis--an inflammatory disease. N Engl J Med. 1999. 340(2):115–126.

13. Palmer RM, Ashton DS, Moncada S. Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature. 1988. 333(6174):664–666.

14. Rajfer J, Aronson WJ, Bush PA, Dorey FJ, Ignarro LJ. Nitric oxide as a mediator of relaxation of the corpus cavernosum in response to nonadrenergic, noncholinergic neurotransmission. N Engl J Med. 1992. 326(2):90–94.

15. Bredt DS, Ferris CD, Snyder SH. Nitric oxide synthase regulatory sites. Phosphorylation by cyclic AMP-dependent protein kinase, protein kinase C, and calcium/calmodulin protein kinase; identification of flavin and calmodulin binding sites. J Biol Chem. 1992. 267(16):10976–10981.

16. Singh KC, Suryanarayna N. Eri pupae: A popular cuisine too. Indian Silk. 2003. 41:57–58.

17. Zhou J, Han DX. Proximate, amino acid and mineral composition of pupae of the silkworm Antheraea pernyi in China. J Food Compos Anal. 2006. 19:850–853.

18. Usub T, Lertsatitthanakorn C, Poomsa-ad N, Wiset L, Yang LF, Siriamornpun S. Experimental performance of a solar tunnel dryer for drying silkworm pupae. Biosyst Eng. 2008. 101:209–216.

19. Wei ZJ, Liao AM, Zhang HX, Liu J, Jiang ST. Optimization of supercritical carbon dioxide extraction of silkworm pupal oil applying the response surface methodology. Bioresour Technol. 2009. 100:4214–4219.

20. Ryu KS, Ahn MY, Lee HS, Kim IK, Kim JW, Kim SH, Choi JH. The tonic effect of the extract from male silkworm (Bombyx mori L.) pupae on rats. Int J Indust Entomol. 2002. 5(1):123–126.

21. Tirapelli CR, Fukada SY, Yogi A, Chignalia AZ, Tostes RC, Bonaventura D, Lanchote VL, Cunha FQ, de Oliveira AM. Gender-specific vascular effects elicited by chronic ethanol consumption in rats: a role for inducible nitric oxide synthase. Br J Pharmacol. 2008. 153(3):468–479.

22. Shinoda M, Latour MG, Lavoie JM. Effects of physical training on body composition and organ weights in ovariectomized and hyperestrogenic rats. Int J Obes Relat Metab Disord. 2002. 26(3):335–343.

23. Fung MM, Bettencourt R, Barrett-Connor E. Heart disease risk factors predict erectile dysfunction 25 years later: the Rancho Bernardo Study. J Am Coll Cardiol. 2004. 43(8):1405–1411.

24. Luc TF. Erectile dysfunction. N Engl J Med. 2000. 342(24):1802–1813.

25. Fridovich I. Superoxide dismutases. Annu Rev Biochem. 1975. 44:147–159.

26. Kono Y, Fridovich I. Superoxide radical inhibits catalase. J Biol Chem. 1982. 257(10):5751–5754.

27. Halliwell B, Gutteridge JM. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J. 1984. 219(1):1–14.

28. Bendich A, D'Apolito P, Gabriel E, Machlin LJ. Interaction of dietary vitamin C and vitamin E on guinea pig immune responses to mitogens. J Nutr. 1984. 114(9):1588–1593.

29. Ross D. Glutathione, free radicals and chemotherapeutic agents. Mechanisms of free-radical induced toxicity and glutathione-dependent protection. Pharmacol Ther. 1988. 37(2):231–249.

30. Hornsby PJ, Crivello JF. The role of lipid peroxidation and biological antioxidants in the function of the adrenal cortex. Part 2. Mol Cell Endocrinol. 1983. 30(2):123–147.

31. Furchegott RF. Role of endothelium in response of vascular smooth muscle. Circ Res. 1983. 53(5):557–573.