Korean J Vet Res.
2012 Mar;52(1):25-31.
Diagnosis of canine brucellosis using recombinant ribosomal protein L7/L12
- Affiliations
-
- 1Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang 430-824, Korea. herm@korea.kr
Abstract
- Brucella (B.) canis is mainly transmitted by direct or indirect contact with aborted fetuses and placenta. It's also known to be able to infect human, which likely results in providing veterinarians and companion animal owners for infectious risk. To develop diagnostic ELISA, we cloned and expressed rp1L gene of B. canis, which encodes the ribosomal protein L7/L12. Using this purified recombinant protein, indirect-ELISA (iELISA) was evaluated using 78 positive and 44 negative sera. The sensitivity and the specificity of iELISA were 94% and 89%, respectively. The results indicated that indirect-ELISA using recombinant ribosomal protein L7/L12 may be useful for diagnosis of canine brucellosis.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Diagnosis of human brucellosis
- Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein
- Acute Lymphoblastic Leukemia Associated with Brucellosis in Two Patients with Fever and Pancytopenia
- Prevalence state of canine brucellosis in South Korea during 2015 and 2016
- In silico analysis of Brucella abortus Omp2b and in vitro expression of SOmp2b
