J Korean Med Sci.  1994 Feb;9(1):16-20. 10.3346/jkms.1994.9.1.16.

Comparison of bromodeoxyuridine and proliferating cell nuclear antigen labeling in gastric carcinoma

• Kang SM
• Kim WH
• Kim CW
• Kim YI

• KMID: 1422181
• DOI: http://doi.org/10.3346/jkms.1994.9.1.16

Abstract

The proliferative activity of human gastric carcinoma was measured by means of in vitro incorporation of the thymidine analogue, 5-bromo-2'-deoxyuridine (BrdU), into the newly-synthesized DNA of fresh tumors and immunohistochemical staining of proliferating cell nuclear antigen (PCNA) using avidin-biotin peroxidase method. Eighty-two cases of surgically resected human gastric carcinomas consisting of 18 various histologic types were subjected to study. The mean BrdU labelling index (LI) and PCNA LI were 22.9% and 39.1%, respectively. The correlation between BrdU LI and PCNA LI was statistically significant (correlation coefficient mu = 0.61334, p = 0.0001). We concluded that immunohistochemical staining for PCNA may become a practical method instead of in vitro or in vivo BrdU labeling to assess the proliferation fraction of the gastric cancer patient.

MeSH Terms

Antibodies, Monoclonal
Antigens, Neoplasm/*metabolism
Bromodeoxyuridine/*metabolism
Carcinoma/*metabolism/pathology
Cell Division
DNA Replication
DNA, Neoplasm/biosynthesis
Humans
Immunoenzyme Techniques
Nuclear Proteins/*metabolism
Proliferating Cell Nuclear Antigen
Stomach Neoplasms/*metabolism/pathology
Tumor Cells, Cultured
Antibodies, Monoclonal
Antigens, Neoplasm
DNA, Neoplasm
Nuclear Proteins
Proliferating Cell Nuclear Antigen
Bromodeoxyuridine