J Korean Med Sci.  1993 Apr;8(2):153-159. 10.3346/jkms.1993.8.2.153.

The mechanism of c-erbB-2 gene product increase in stomach cancer cell lines

• Bae CD
• Park SE
• Seong YS
• Kim SW
• park JB
• Park JG

• KMID: 1421967
• DOI: http://doi.org/10.3346/jkms.1993.8.2.153

Abstract

c-erbB-2 oncogene encodes a growth factor receptor whose amino acid sequence has extensive homology with human epidermal growth factor receptor. It is frequently overexpressed in human breast, ovary, lung, and stomach cancers, where its overexpression is related significantly to the prognosis. Tl investigate the possible role of c-erbB-2 oncogene in the oncogenesis of stomach cancer, we examined the genetic alterations of c-erbB-2 oncogene in 4 stomach cancer cell lines, SNU-1, SNU-5, SNU-16 and KATO III. There were no differences in c-erbB-2 mRNA level as well as c-erbB-2 gene copy number among them. But gp185-erbB-2, c-erbB-2 gene product, was increased from 2- to 4-fold in SNU-1 and SNU-5 cells, compared with that in SNU-16 or KATO III cells. Our results suggest that post-transcriptional regulation of gp185erbB-2 expression may underlie gp185erbB-2 overexpression in cancer cells.

Keyword

c-erbB-2 oncogene-stomach cancer-post-transcriptional regulation

MeSH Terms

Amino Acid Sequence
Humans
Molecular Sequence Data
Protein-Tyrosine Kinases/*biosynthesis
Proto-Oncogene Proteins/*biosynthesis/genetics/immunology
RNA, Messenger/analysis
Receptor, Epidermal Growth Factor/*biosynthesis/genetics/immunology
Receptor, erbB-2
Receptors, Cell Surface/*biosynthesis
Stomach Neoplasms/genetics/*metabolism
Tumor Cells, Cultured
Proto-Oncogene Proteins
RNA, Messenger
Receptors, Cell Surface
Protein-Tyrosine Kinases
Receptor, Epidermal Growth Factor
Receptor, erbB-2