J Korean Med Sci.  1992 Mar;7(1):40-46. 10.3346/jkms.1992.7.1.40.

The effect of hyperglycemia on lipid peroxidation in the global cerebral ischemia of the rat

• Roh JK
• Hong SB
• Yoon BW
• Kim MS
• Myung HJ

• KMID: 1421888
• DOI: http://doi.org/10.3346/jkms.1992.7.1.40

Abstract

To investigate the influence of hyperglycemia on ischemic brain damage, we measured brain ATP, lactate and malondialdehyde (MDA) levels in global cerebral ischemic models of Wistar rats. We induced global cerebral ischemia by the 4-vessel occlusion method. After 30 or 60 min of occlusion, and after 30 min of reperfusion, we measured brain ATP, lactate and MDA levels. During the ischemic period, brain ATP levels decreased to 30-70% of sham groups both in normoglycemic and hyperglycemic groups. But during the reperfusion period, the recovery rate of ATP levels was significantly lower in the hyperglycemic than in the normoglycemic groups (p less than 0.05). After 60 min of global ischemia, brain lactate increased much more in the hyperglycemic than in the normoglycemic group, and, during reperfusion, was washed out slowly in the hyperglycemic group. The MDA level, a parameter of lipid peroxidation, increased more in the hyperglycemic group than in the normoglycemic group during reperfusion periods (p less than 0.05). We conclude that hyperglycemia increases lactate accumulation, delays the recovery of energy metabolism, and enhances the lipid peroxidation in the transient global ischemia of rat brain. These findings may suggest the harmfulness of hyperglycemia in clinical cerebral ischemia.

Keyword

Global Cerebral Ischemia; ATP; Lactate; MDA; Lipid Peroxidation

MeSH Terms

3,4-Methylenedioxyamphetamine/metabolism
Adenosine Triphosphate/metabolism
Animals
Hyperglycemia/complications/*metabolism
Ischemic Attack, Transient/complications/*metabolism
Lactates/metabolism
*Lipid Peroxidation
Male
Rats
Rats, Wistar
Lactates
3,4-Methylenedioxyamphetamine
Adenosine Triphosphate