J Korean Acad Nurs.  2010 Oct;40(5):611-619. 10.4040/jkan.2010.40.5.611.

Effects of Exercise on Affected and Unaffected Hindlimb Muscles in Rats with Neuropathic Pain Induced by Unilateral Peripheral Nerve Injury

• Choe MA
• An GJ

• KMID: 1394852
• DOI: http://doi.org/10.4040/jkan.2010.40.5.611

Abstract

PURPOSE
The purpose of this study was to examine the effects of exercise on muscle weight and Type I and II fiber cross-sectional area of affected and unaffected hindlimb muscles in rats with neuropathic pain induced by unilateral peripheral nerve injury.
METHODS
Neuropathic pain was induced by ligation and cutting of the left L5 spinal nerve. Adult male Sprague-Dawley rats were randomly assigned to one of two groups: The Pain+Exercise (PE) group (n=21) and the Sham+Exercise (SE) group (n=20). All rats had 28 sessions of treadmill exercise at grade 10 for 30 minutes, twice/day at 10 m/min for 14 days. Body weight, food intake and activity were measured every day. At 15 days all rats were anesthetized and soleus, plantaris and gastrocnemius muscles were dissected. Muscle weight and Type I, II fiber cross-sectional area of the dissected muscles were measured.
RESULTS
The PE group showed significant increases (p<.05), as compared to the SE group for body weight and total diet intake, muscle weight of the unaffected soleus and plantaris, and in Type I and II fiber cross-sectional area of unaffected three muscles and affected plantaris.
CONCLUSION
Exercise for 14 days attenuates unaffected soleus, plantaris and gastrocnemius muscle atrophy in neuropathic pain model.

Keyword

Pain; Exercise; Muscular atrophy; Muscle weight; Muscle fiber

MeSH Terms

Animals
Body Weight
Disease Models, Animal
Eating
Hindlimb/*physiology
Male
Muscle Fibers, Skeletal/*physiology
Muscular Atrophy/etiology/physiopathology
Neuralgia/*etiology
Peripheral Nerves/*injuries
*Physical Conditioning, Animal
Rats
Rats, Sprague-Dawley

Figure

• 

  Figure 1 Cross-section of the hindlimb muscles in Pain+Exercise and Sham+Exercise rats. The first line is the affected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Pain+Exercise rats. The second line is the unaffected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Pain+Exercise rats. The third line is the affected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Sham+Exercise rats. The fourth line is the unaffected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Sham+Exercise rats. Dark=Type I muscle fiber, light=Type II muscle fiber (Myosin ATPase straining, 100×).

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