J Vet Sci.  2012 Jun;13(2):193-197. 10.4142/jvs.2012.13.2.193.

A modified method for inducing periodontitis in dogs using a silk-wire twisted ligature

• Kim SE
• Lee ER
• Lee Y
• Jeong M
• Park YW
• Ahn JS
• Ahn JT
• Seo K

• KMID: 1376194
• DOI: http://doi.org/10.4142/jvs.2012.13.2.193

Abstract

This study was designed to assess the effectiveness of a modified silk ligature twisted with wire for inducing advanced periodontitis. Periodontitis was induced in five premolars and one molar of 20 healthy dogs over a 60-day period. The dogs were divided into four groups according to the ligature-inducing materials used: soft moistened food only, wire ligature (WL), silk ligature (SL) and twisted ligature with silk and wire (SWL). Periodontal indices were recorded, and dental radiographs were taken before and after 60 days of ligation. The ligatures were checked daily and the day the ligature fell out was noted. The period during which the ligatures were maintained was significantly shorter for the SL group compared to the SWL group (p < 0.05). Results of the clinical examination showed that almost all periodontal status parameters including the plaque index, gingival index, clinical attachment level, and bleeding on probing were significantly exacerbated in the SWL group compared to the other groups (p < 0.05). Radiographic evaluation demonstrated that alveolar bone levels were significantly lower in the SWL group than the other groups on day 60 (p < 0.05). These results suggested that experimental periodontitis induced by SWL could be an effective method for investigating periodontitis in canine models.

Keyword

dog; experimental model; periodontitis

MeSH Terms

Alveolar Bone Loss/veterinary
Analgesics, Opioid/therapeutic use
Animals
Dog Diseases/*pathology
Dogs
Ligation/instrumentation/methods/*veterinary
Materials Testing/veterinary
Pain/drug therapy/veterinary
Periodontitis/pathology/*veterinary
Tramadol/therapeutic use

Figure

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  Fig. 1 Ligature materials. (A) 0.012-inch ligature wire, (B) 2-0 braided silk, (C) twisted ligature with silk and wire.

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  Fig. 2 Alveolar bone levels before (day 0) and 60 days after (day 60) ligation in each group. a,b,c,dValues with different superscript letters were significantly different on the same day (p = 0.000). *Significantly decreased compared to day 0 in the same group (p < 0.05). ABL: alveolar bone level, SF: negative control, WL: wire ligature, SL: silk ligature, SWL: silk-wire twisted ligature.

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