Exp Mol Med.
2004 Oct;36(5):396-410.
Evaluation of biological monitoring markers using genomic and proteomic analysis for automobile emission inspectors and waste incinerating workers exposed to polycyclic aromatic hydrocarbons or 2,3,7,8,-tetracholrodedibenzo-p-dioxins
- Affiliations
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- 1Department of Biochemistry and Molecular Biology, School of Medicine, Korea University, Anamdong 5-ga, Sungbuk-gu, Seoul 136-705, Korea.
- 2Graduate School of Biotechnology, Korea University, Anamdong 5-ga, Sungbuk-gu, Seoul 136-705, Korea.
- 3School of Public Health, Korea University, Anamdong 5-ga, Sungbuk-gu, Seoul 136-705, Korea.
- 4Department of Preventive Medicine School of Medicine and Institute for Environmental Health Medical Science Research Center.
- 5Environmental Toxico-Genomic and Proteomic Center, School of Medicine, Korea University, Anamdong 5-ga, Sungbuk-gu, Seoul 136-705, Korea. dsul@korea.ac.kr
Abstract
- In this study, we investigated the effects of PAHs and dioxin on mRNA and plasma protein expression using genomic and proteomic analysis for automobile emission inspectors and waste incineration workers. About 54 workers from automobile emission inspection offices, 31 workers from waste incinerating company and 84 unexposed healthy subjects were enrolled in this study. Urine and air samples were collected and analyzed by HPLC and GC/MS. Comet assays were carried out to evaluate any DNA damage in mononuclear and polynuclear cells. A significant difference in Olive tail moments in mononuclear cells was observed between exposed and control subjects (P <0.0001). To examine the differences of the gene expression profile in automobile emission inspectors and waste incineration workers, radioactive complementary DNA microarrays were used to evaluate changes in the expression of 1,152 total genes. The gene expression profiles showed that 11 genes were up-regulated and 4 genes were down-regulated in waste incinerating workers as compared with controls. Plasma proteins were analyzed by 2-dimentional electrophoresis with pH 3-10 NL IPG Dry strip. The protein expression profiles showed that 8 proteins were up- regulated and 1 protein, haptoglobin, was down- regulated in automobile emission inspectors and waste incineration workers. Serum paraoxonase/ arylesterase was found only in the plasma of waste incineration workers. The expression of genes and proteins involved in oxidative stress were up-regulated in both automobile emission inspectors and waste incineration workers. Several proteins, such as transthyrethin, sarcolectin and haptoglobin, that were highly up- or down-regulated, could serve as biological monitoring markers for future study.
Keyword
MeSH Terms
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Adult
Aged
Biological Markers/analysis
DNA Fragmentation
Environmental Monitoring/*methods
Gene Expression Profiling
Genetic Markers
Genomics
Humans
*Incineration
Middle Aged
Naphthols/urine
Occupational Exposure/analysis
Oligonucleotide Array Sequence Analysis
Polycyclic Hydrocarbons, Aromatic/analysis/*toxicity
Proteomics
Pyrenes/analysis
Research Support, Non-U.S. Gov't
Tetrachlorodibenzodioxin/analysis/*toxicity
*Vehicle Emissions
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