J Korean Med Sci.
2002 Aug;17(4):483-489. 10.3346/jkms.2002.17.4.483.
Immunoreactivity of CD99 in Stomach Cancer
- Affiliations
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- 1Department of Pathology, College of Medicine, Hallym University, Chunchon, Korea. jungkc@hallym.ac.kr
- 2Department of Pathology, College of Medicine, Kangwon National University, Chunchon, Korea.
- 3Department of Anatomy, College of Medicine, Kangwon National University, Chunchon, Korea.
- 4Vascular System Research Center, Kangwon National University, Chunchon, Korea.
- 5Division of Life Sciences, College of Natural Science, Kangwon National University, Chunchon, Korea.
- 6Department of Pathology, Seoul National University College of Medicine, Seoul, Korea.
- KMID: 1109756
- DOI: http://doi.org/10.3346/jkms.2002.17.4.483
Abstract
- CD99 is characteristically expressed in Ewing's sarcoma/primitive neuroectodermal tumor. Recently its immunoreactivity has also been reported in other tumors. However, the significance of CD99 isoforms expressed in these tumors has not been elucidated. In this study, we evaluated the expression of CD99 isoforms and its relationship with histopathologic parameters in gastric adenocarcinomas. Paraffin sections of 46 gastric adenocarcinomas were stained with an anti-CD99 monoclonal antibody, YG32. Twelve (26.1%) cases of 46 gastric adenocarcinomas showed immunoreactivity to YG32. The CD99 expression was also seen both in non-neoplastic foveolar epithelial cells and infiltrating lymphocytes. In addition, Western blot and RT-PCR analyses revealed that the type I is the predominant isoform of CD99 in non-neoplastic and neoplastic gastric tissues. The CD99 expression was usually seen in the intestinal type adenocarcinoma, while rarely in the diffuse type. The CD99 immunoreactivity decreased in MMP-2-overexpressing adenocarcinomas (p=0.028). Our results suggest that the type I is the major isoform of CD99 expressed in non-neoplastic gastric mucosa and gastric adenocarcinomas and its downregulation in gastric adenocarcinoma may be associated with cellular dedifferentiation and/or MMP-2 overexpression.
MeSH Terms
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Adenocarcinoma/*immunology/pathology
Adult
Aged
Antigens, CD/*analysis/genetics
Cell Adhesion Molecules/*analysis/genetics
Female
Gastric Mucosa/cytology/immunology
Humans
Male
Matrix Metalloproteinases/metabolism
Middle Aged
Protein Isoforms/analysis/genetics
RNA, Messenger/genetics/metabolism
Stomach Neoplasms/*immunology/pathology
Cited by 2 articles
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Expression of CD99 in Pleomorphic Carcinomas of the Lung
Seong-Ho Yoo, Jungho Han, Tae Jin Kim, Doo Hyun Chung
J Korean Med Sci. 2005;20(1):50-55. doi: 10.3346/jkms.2005.20.1.50.Immunoreactivity of CD99 in Non-Hodgkin's Lymphoma: Unexpected Frequent Expression in ALK-positive Anaplastic Large Cell Lymphoma
Chang Ohk Sung, Young H. Ko, Sanghui Park, Kihyun Kim, Wonseog Kim
J Korean Med Sci. 2005;20(6):952-956. doi: 10.3346/jkms.2005.20.6.952.
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