J Korean Med Sci.  1999 Dec;14(6):600-606. 10.3346/jkms.1999.14.6.600.

High CD99 expression in memory T and B cells in reactive lymph nodes

• Park CK
• Shin YK
• Kim TJ
• Park SH
• Ahn GH

• KMID: 646034
• DOI: http://doi.org/10.3346/jkms.1999.14.6.600

Abstract

We investigated the expression of CD99 in 35 hyperplastic perigastric lymph nodes, which were resected for gastric carcinoma or chronic peptic ulcer. Essentially, all lymphocytes in lymph nodes expressed CD99, but there were two populations with respect to the intensity of CD99 expression--CD99high and CD99low cells. We showed CD99high cells were distributed in paracortical and medullary cords by immunohistochemical study while germinal center cells were CD99low. Using three-color flow cytometric analysis with CD3, CD4, CD8, CD19, CD23, CD45RA, CD45RO, CD69, CD138, IgM, IgD, and IgG, most of CD99high cells were shown to be activated/memory T cells. CD4+CD45RO+ T cells were the subset revealing the highest intensity of CD99 expression while CD4+CD45RA+ T cells were CD99low. Among B cells, IgG+ B cells revealed a higher level of CD99 molecules than IgM+ B cells. These results suggest that CD99 is one of activation-related molecules which are upregulated in recently activated lymphocytes.

Keyword

Immunohistochemistry; Lymphocyte transformation; Memory

MeSH Terms

Adult
Aged
Antigens, CD/analysis*
B-Lymphocytes/immunology*
Cell Adhesion Molecules/analysis*
Flow Cytometry
Germinal Center/immunology
Human
Immunohistochemistry
Immunologic Memory/immunology*
Lymph Nodes/immunology*
Middle Age
Peptic Ulcer/immunology*
Stomach Neoplasms/immunology*
T-Lymphocytes/immunology*