Korean J Parasitol.  2010 Dec;48(4):285-290. 10.3347/kjp.2010.48.4.285.

Involvement of Src Family Tyrosine Kinase in Apoptosis of Human Neutrophils Induced by Protozoan Parasite Entamoeba histolytica

• Sim S
• Yu JR
• Lee YA
• Shin MH

• KMID: 1104693
• DOI: http://doi.org/10.3347/kjp.2010.48.4.285

Abstract

Tyrosine kinases are one of the most important regulators for intracellular signal transduction related to inflammatory responses. However, there are no reports describing the effects of tyrosine kinases on neutrophil apoptosis induced by Entamoeba histolytica. In this study, isolated human neutrophils from peripheral blood were incubated with live trophozoites in the presence or absence of tyrosine kinase inhibitors. Entamoeba-induced receptor shedding of CD16 and PS externalization in neutrophils were inhibited by pre-incubation of neutrophils with the broad-spectrum tyrosine kinase inhibitor genistein or the Src family kinase inhibitor PP2. Entamoeba-induced ROS production was also inhibited by genistein or PP2. Moreover, genistein and PP2 blocked the phosphorylation of ERK and p38 MAPK in neutrophils induced by E. histolytica. These results suggest that Src tyrosine kinases may participate in the signaling event for ROS-dependent activation of MAPKs during neutrophil apoptosis induced by E. histolytica.

Keyword

Entamoeba histolytica; neutrophil; apoptosis; Src tyrosine kinase

MeSH Terms

*Apoptosis
Cells, Cultured
Entamoeba histolytica/*immunology/*pathogenicity
GPI-Linked Proteins/metabolism
Genistein/metabolism
Humans
Neutrophils/*immunology
Protein Kinase Inhibitors/metabolism
Pyrimidines/metabolism
Reactive Oxygen Species/metabolism
Receptors, IgG/metabolism
src-Family Kinases/antagonists & inhibitors/*metabolism