J Vet Sci.  2006 Mar;7(1):73-77. 10.4142/jvs.2006.7.1.73.

Guided bone regeneration with beta-tricalcium phosphate and poly Llactide-co-glycolide-co-epsilon-caprolactone membrane in partial defects of canine humerus

• Oh T
• Rahman MM
• Lim JH
• Park MS
• Kim DY
• Yoon JH
• Kim WH
• Kikuchi M
• Tanaka J
• Koyama Y
• Kweon OK

• KMID: 1103558
• DOI: http://doi.org/10.4142/jvs.2006.7.1.73

Abstract

This study was performed to evaluate the effect of betatricalcium phosphate and poly L-lactide-co-glycolide-coepsilon- caprolactone (TCP/PLGC) membrane in the repair of partial bone defects in canine proximal humerus. Three adult mixed-breed dogs were used during the experimental period. The length of the defect was quarter of the full length of humerus, and width of the defect was quarter of middle diameter of the lateral aspect of humerus. The humeri of each dog were divided into treatment (TCP/ PLGC) and control groups. The defect was covered with TCP/PLGC membrane in treatment group. To evaluate regeneration of the bone, computerized tomography (CT) and histopathologic examination were performed. The radiopaque lines were appeared at the original defect sites in TCP/PLGC group but below the original site in control at 4th week. Radiopacity and thickness of the defect sites, and radiopaque lines were more increased at 8th week than those of 4th week. Histopathologic findings revealed fibrous connective tissue migration into the defect and the migration inhibited the structure of new cortex to be placed in the original level in control whereas new cortex growth was found in the level of original line in TCP/ PLGC group. However, the new cortical bone in the TCP/ PLGC group was thinner and less organized than the adjacent intact cortex, and the amount of new cancellous bones were also scanty. The result suggested that TCP/ PLGC membrane is a good guided bone regeneration material to restore the original morphology of humerus in partial defect.

Keyword

TCP/PLGC membrane; bone; defect; dog

MeSH Terms

Absorbable Implants/veterinary
Animals
Bone Regeneration/*drug effects
Calcium Phosphates/*pharmacology
Dogs/*surgery
Guided Tissue Regeneration/methods/*veterinary
Histocytochemistry/veterinary
Humerus/*surgery
Male
Polyesters/*pharmacology
Tomography, X-Ray Computed/veterinary
Wound Healing/physiology

Figure

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  Fig. 1 Computerized tomography in the transverse planes of the middle of the defects at 4th week after surgery. Radiopaque lines appeared on bone defect area in both groups, control group (L1-3) and TCP/PLGC group (R1-3).

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  Fig. 2 Computerized tomography at the transverse planes of the middle of the defects at 8th week after surgery. Radiopaque lines were found in the same site of the 4th week scan, but their thickness and radiopacity were increased. L1-3 (Control group) and R1-3 (TCP/PLGC group).

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  Fig. 3 Histopathologic findings of the bone defect in the control group at 8th week. Active periosteal reaction was observed, but the degree of cancellous bone formation is less prominent than that of adjacent intact area. (arrow: new regenerated bone; arrow-head: intact bone). H&E stain, ×40.

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  Fig. 4 Histopathologic findings of the bone defect in the TCP/PLGC group at 8th week. Active periosteal reaction was observed. The new cortex was recovered up to the original level. Regenerated cancellous bone seemed to be less amount compared with adjacent intact cancellous bone. (arrow: new regenerated bone; arrow-head: intact bone). H&E stain. ×40.

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