Exp Mol Med.
2004 Dec;36(6):576-581.
Different inhibition characteristics of intracellular transglutaminase activity by cystamine and cysteamine
- Affiliations
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- 1Department of Biochemistry and Molecular Biology/Aging Apoptosis Research Center (AARC) Seoul National University College of Medicine Seoul 110-799, Korea. igkim@plaza.snu.ac.kr
Abstract
- The treatment of cystamine, a transglutaminase (TGase) inhibitor, has beneficial effects in several diseases including CAG-expansion disorders and cataract. We compared the inhibition characteristics of cystamine with those of cysteamine, a reduced form of cystamine expected to be present inside cells. Cystamine is a more potent inhibitor for TGase than cysteamine with different kinetics pattern in a non- reducing condition. By contrast, under reducing conditions, the inhibitory effect of cystamine was comparable with that of cysteamine. However, cystamine inhibited intracellular TGase activity more strongly than cysteamine despite of cytoplasmic reducing environment, suggesting that cystamine itself inhibits in situ TGase activity by forming mixed disulfides.
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