J Vet Sci.  2004 Sep;5(3):241-245.

Repeated dose toxicity of alfa-cypermethrin in rats

• Manna S
• Bhattacharyya D
• Mandal TK
• Das S

Abstract

The present study was performed to investigate the subacute effect of alpha-cypermethrin (alpha-CP) in rats. Alfacypermethrin a synthetic pyrethroid insecticide, dissolved in dimethyl sulfoxide (DMSO) and oral LD50 was investigated after administering orally different doses in rats and was determined as 145 mg/kg. Other groups of rats were given repeated daily oral dose (1/10 LD50) of alpha-CP for 30 days. The animals were sacrificed on 31st day. Activities of various enzymes, cytochrome P450 and b5 contents in liver, hepatic antioxidant status, tissue residue concentration, haemogram and pathological changes were studied. It increased the serum aminotransaminases (AST, ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) activities and blood glucose level significantly. alpha-CP decreased RBC count, PCV and Hb level significantly. It significantly decreased cytochrome P450 in liver. Residues were present in different tissues. It increased malondialdehyde (MDA) level, while decreased the activities of catalase (CAT), superoxide dismutase (SOD) and glycogen level in liver significantly. Mild to moderate histological alterations were observed in lungs, liver, stomach, kidneys, testes and cerebellum. So repeated daily oral doses of alpha-CP at 1/10LD50 altered the biochemical parameters, decreased cytochrome P450 content, antioxidant status, which correlated with histopathological changes of tissues.

Keyword

alpha -CP; cytochrome P450; cytochrome b5; antioxi-dants; tissue residue concentration; histopathology; rat

MeSH Terms

Alanine Transaminase/blood
Animals
Aspartate Aminotransferases/blood
Blood Glucose/metabolism
Catalase/metabolism
Cytochrome P-450 Enzyme System/metabolism
Erythrocyte Count/veterinary
Female
Glycogen/metabolism
Hematocrit/veterinary
Hemoglobins/metabolism
Insecticides/*toxicity
Kidney/drug effects/metabolism/pathology
L-Lactate Dehydrogenase/blood
Lethal Dose 50
Liver/drug effects/metabolism/pathology
Lung/drug effects/metabolism/pathology
Male
Malondialdehyde/metabolism
Pyrethrins/*toxicity
Rats
Rats, Wistar
Superoxide Dismutase/metabolism
Testis/drug effects/metabolism/pathology