Exp Mol Med.
2006 Oct;38(5):485-493.
Identification of a novel murine organic anion transporter like protein 1 (OATLP1) expressed in the kidney
- Affiliations
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- 1Department of Pharmacology and Toxicology, and Center for Advanced Medical Education by BK21 Project Inha University, Incheon, Korea. shcha@inha.ac.kr
- 2Department of Laboratory Animal Medical Research Center, College of Medicine, Yonsei University, Seoul, Korea.
- 3Department of Anatomy and Cell Death Disease Research Center for MRC, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Abstract
- The organic anion transporters (OATs) are expressed in various tissues, primarily in the kidney and liver, but they are also expressed in the placenta, small intestine, and the choroid plexus, which are all epithelial tissues that transport xenobiotics. Six isoforms of OATs are currently known. Considering the variety of organic anionic compounds, other OATs isoforms can be assumed. In this connection, we have searched for a new isoform in the expressed sequence tag (EST) database. We found the new candidate clone AK052752 in the mouse kidney cDNA library and we named it mouse organic anion transporter like protein 1 (mOATLP1). The mOATLP1 cDNA consisted of 2221 base pairs that encoded a 552 amino acid residue protein with 12 putative transmembrane domains. The deduced amino acid sequence of mOATLP1 showed 37 to 63% identity to other members of the OAT family. According to the tissue distribution based on Northern blot analysis, 2.7 kb and 2.9 kb mOATLP1 transcripts (approximate sizes) were observed in the kidney and liver. An 85-kDa band (approximate) was detected using Western blot analysis of mouse kidney performed with a synthesized oligopeptide-induced mOATLP1 antibody. Immunohistochemical results showed mOATLP1 was stained in the blood vessels, glomeruli (the parietal epithelial cells and podocytes), distal convoluted tubules, connecting tubules, and inner medullary collecting tubules. mOATLP1 appears to be a novel candidate for an organic anion transporter isoform identified in the kidney.
Keyword
MeSH Terms
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Tissue Distribution
Sequence Homology, Amino Acid
Rabbits
Protein Structure, Tertiary
Protein Isoforms/isolation & purification
Phylogeny
Organic Anion Transporters/*isolation & purification/*metabolism
Oligopeptides/immunology
Multigene Family
Molecular Sequence Data
Mice
Kidney/*metabolism
Immunohistochemistry
Cloning, Molecular
Blotting, Western
Animals
Amino Acid Sequence
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