Exp Mol Med.  2002 Mar;34(1):83-89.

Effects of chlorinated aliphatic hydrocarbons on the fidelity of cell division in human CYP2E1 expressing cells

• Kim A
• Parry EM
• Parry JM

Abstract

Chlorinated organic chemicals are widely used in industry and are present in the environment. Five chlorinated aliphatic hydrocarbons, namely 1-2-dichloroethane, 1,1,2-trichloroethane, trichloroethylene, 2,3-dichlorobutane and 1-chlorohexane were investigated to determine their influence upon the fidelity of cell division in cultured mammalian cells. In order to determine the influence of these chemical compounds upon the fidelity of cell division, a technique known as differential staining of chromosomes and spindle was performed with one genetically engineered cell line and its parental cell line. The genetically engineered cell line used in this study expressed a human P450 enzyme, CYP2E1. Four chemicals, 1-2-dichloroethane, trichloroethylene, 2,3-dichlorobutane and 1-chlorohexane required metabolic bioactivations in order to induce spindle damage in cultured mammalian cells whereas 1,1,2-trichloroethane was a direct-acting spindle poison.

MeSH Terms

Animal
Cell Division/*drug effects
Cell Line
Chromosomes/metabolism
Cytochrome P-450 CYP2E1/genetics/*metabolism
Enzyme Activation
Hamsters
Human
Hydrocarbons, Chlorinated/chemistry/*pharmacology
Mitotic Spindle Apparatus
Staining and Labeling/methods