Exp Mol Med.
2005 Aug;37(4):343-352.
Methyl gallate and chemicals structurally related tomethyl gallate protect human umbilical vein endothelial cells from oxidative stress
- Affiliations
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- 1College of Pharmacy, Chung-Ang University, 221 Heukseok-dong, Dongjak-gu, Seoul 156-755, Korea.
- 2KeyGene Life Science Institute, KeyGene Science, Corp, Ansan, Gyeonggi-do 425-791, Korea.
- 3Laboratory of Molecular Genetics, Research Institute of Medical Science, College of Medicine, The Catholic University of Korea, Seoul 137-040, Korea. jinwoo@catholic.ac.kr
- 4Department of Obstetrics and Gynecology, College of Medicine, The Catholic University of Korea, Seoul 137-040, Korea.
- 5Department of Biostatistics, College of Medicine, The Catholic University of Korea, Seoul 137-040, Korea.
- 6Department of Biochemistry and Molecular Biology, Aging-assoiciated Vascular Disease Research Center, College of Medicine, Yeungnam University, Daegu 705-717, Korea.
Abstract
- Methyl gallate (meGAL) is known as one of major antioxidants. To investigate whether meGAL protects human cells from oxidative stress, meGAL extracted from Korean medicinal plant, Cercis chinensis leaves, was primarily screened using cell viability assay against oxidative stress. Human umbilical vein endothelial cells (HUVECs) were treated with three different concentrations of meGAL for indicated time. After or during meGAL treatment, H2O2 was added and incubated. meGAL showed free radical scavenging effect at low concentration (0.02 mM) and cell protective effect against H2O2-mediated oxidative stress. meGAL recovered viability of HUVECs damaged by H2O2-treatment, reduced the lipid peroxidation (LPO) and decreased the internal reactive oxygen species (ROS) level elevated by H2O2-treatment. Free radical scavenging effect of meGAL was proven to be very high. Differential display reverse transcription-PCR analysis showed that meGAL upregulated the levels of regulator of chromatin condensation 1, type 1 sigma receptor and phosphate carrier protein expressions, respectively. Based on structural similarity compared with meGAL, 14 chemicals were chosen and viability assay was performed. Four chemicals, haematommic acid (56.2% enhancement of viability), gallic acid (35.0%), methylorsellinic acid (23.7%), and syringic acid (20.8%), enhanced more potent cell viability than meGAL, which showed only 18.1% enhancement of cell viability. These results suggest that meGAL and four meGAL-related chemicals protect HUVECs from oxidative stress.
Keyword
MeSH Terms
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Antioxidants/*chemistry/*pharmacology
Biological Assay
Catalase/analysis
Endothelial Cells/*drug effects/enzymology
Fabaceae/*metabolism
Free Radical Scavengers/chemistry/pharmacology
Gallic Acid/*analogs & derivatives/chemistry/pharmacology
Gene Expression/drug effects
Humans
Molecular Structure
Oxidative Stress/*drug effects/genetics
Plant Extracts/chemistry/pharmacology
Plant Leaves/metabolism
Research Support, Non-U.S. Gov't
Superoxide Dismutase/analysis
Umbilical Veins/cytology
Water/pharmacology
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