J Vet Sci.  2007 Sep;8(3):295-297. 10.4142/jvs.2007.8.3.295.

Distribution of caveolin isoforms in the lemur retina

• Berta AI
• Kiss AL
• Lukats A
• Szabo A
• Szel A

• KMID: 1090806
• DOI: http://doi.org/10.4142/jvs.2007.8.3.295

Abstract

The distribution of caveolin isoforms was previouslyevaluated in the retinas of different species, but has notyet been described in the primate retina. In this study, thedistribution of caveolins was assessed via immunochemistryusing isoform-specific antibodies in the retina of the black-and-white ruffed lemur. Here, we report the presence of avariety of caveolin isoforms in many layers of the lemurretina. As normal human retinas were not available forresearch and the retinas of primates are fairly similar tothose of humans, the lemur retina can be utilized as amodel for caveolin distribution in normal humans.

Keyword

caveolin; immunocytochemistry; lemur; retina

MeSH Terms

Animals
Caveolins/*metabolism
Immunohistochemistry
Lemur/*metabolism
Male
Protein Isoforms
Retina/*metabolism

Figure

• 

  Fig. 1 Immunocytochemistry analysis of caveolin-1, -2 and -3 in the lemur retina. Samples were obtained from different anatomical sites of the retina following the radial plane from the central to the peripheral retina. Alexa Fluor 488 was used to detect caveolins (green, arrows). The cytoskeleton and the nuclei were marked with Alexa Fluor 594 labelled phalloidin (red) and 4,6-diamidino-2-phenylindole (blue), respectively. Merged images are shown. Caveolin-1 was observed in all layers with the same density among the layers at the macular region and the periphery. At the ciliary body, caveolin-1 stained both layers. Hardly any caveolin-2 signals were observed, and were detected only in the vascular cells of the central portion and the peripheral region. No signs were observed in the ciliary body. Caveolin-3 was present in all layers. OS: outer segments, IS: inner segments, ONL: outer nuclear layer, OPL: outer plexiform layer, INL: inner nuclear layer, IPL: inner plexiform layer, GL: ganglion layer, OL: outer layer, IL: inner layer, BV: blood vessel, bar = 10 µm.

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