J Vet Sci.  2007 Sep;8(3):243-247. 10.4142/jvs.2007.8.3.243.

Antimicrobial susceptibility and molecular detection of chloramphenicol and florfenicol resistance among Escherichia coli isolates from diseased chickens

• Li XS
• Wang GQ
• Du XD
• Cui BA
• Zhang SM
• Shen JZ

• KMID: 1090798
• DOI: http://doi.org/10.4142/jvs.2007.8.3.243

Abstract

Seventy Escherichia coli isolates recovered from diseasedchickens diagnosed with colibacillosis in Henan Province,China, between 2004 and 2005 were characterized forantimicrobial susceptibility profiles via a broth doublingdilution method. Overall, the isolates displayed resistanceto trimethoprim-sulfamethoxazole (100%), oxytetracycline(100%), ampicillin (83%), enrofloxacin (83%), and ciprofloxacin(81%), respectively. Among the phenicols, resistance wasapproximately 79% and 29% for chloramphenicol andflorfenicol, respectively. Molecular detection revealed thatthe incidence rates of the floR, cmlA, cat1, cat2 and cat3were 29, 31, 16, 13, and 0%, respectively. Additionally,10% of the isolates were positive for both floR and cmlA.As these antimicrobial agents may potentially inducecross-resistance between animal and human bacterialpathogens, their prudent use in veterinary medicine ishighly recommended.

Keyword

antimicrobial resistance; Escherichia coli; flo-rfenicol

MeSH Terms

Animals
Anti-Bacterial Agents/*pharmacology
*Chickens
China/epidemiology
Chloramphenicol/pharmacology
DNA, Bacterial/chemistry/genetics
Drug Resistance, Multiple, Bacterial
Escherichia coli/*drug effects/growth & development
Escherichia coli Infections/epidemiology/microbiology/*veterinary
Microbial Sensitivity Tests/veterinary
Polymerase Chain Reaction/veterinary
Poultry Diseases/epidemiology/*microbiology
Thiamphenicol/analogs & derivatives/pharmacology

Figure

• 

  Fig. 1 Amplification of genes encoding for florfenicol and chloramphenicol resistance determinants. (A) The amplification of the floR genes. Lane 1: Marker; Lane 2: The negative isolate; Lane 3: The positive isolate; Lane 4: The positive control (B) The amplification of the cmlA genes. Lane 1: Marker; Lane 2-3: The positive isolate; Lane 4: The positive control. (C) The amplification of the cat-1 genes. Lane 1: Marker; Lane 2: The positive isolate; Lane 3-4: The negative isolates. (D) The amplification of the cat-2 genes. Lane 1: Marker; Lane 2-3: The positive isolate; Lane 4: The positive control.

Reference

1. Altekruse SF, Elvinger F, Lee KY, Tollefson LK, Pierson EW, Eifert J, Sriranganathan N. Antimicrobial susceptibilities of Escherichia coli strains from a turkey operation. J Am Vet Med Assoc. 2002. 221:411–416.

2. Arcangioli MA, Leroy-Setrin S, Martel JL, Chaslus-Dancla E. A new chloramphenicol and florfenicol resistance gene flanked by two integron structures in Salmonella typhimurium DT104. FEMS Microbiol Lett. 1999. 174:327–332.

3. Arcangioli MA, Leroy-Setrin S, Martel JL, Chaslus-Dancla E. Evolution of chloramphenicol resistance, with emergence of cross-resistance to florfenicol, in bovine Salmonella Typhimurium strains implicates definitive phage type (DT) 104. J Med Microbiol. 2000. 49:103–110.

4. Bass L, Liebert CA, Lee MD, Summers AO, White DG, Thayer SG, Maurer JJ. Incidence and characterization of integrons, genetic elements mediating multiple-drug resistance, in avian Escherichia coli. Antimicrob Agents Chemother. 1999. 43:2925–2929.

5. Bischoff KM, White DG, McDermott PF, Zhao S, Gaines S, Maurer JJ, Nisbet DJ. Characterization of chloramphenicol resistance in beta-hemolytic Escherichia coli associated with diarrhea in neonatal swine. J Clin Microbiol. 2002. 40:389–394.

6. Blanco JE, Blanco M, Mora A, Blanco J. Prevalence of bacterial resistance to quinolones and other antimicrobials among avian Escherichia coli strains isolated from septicemic and healthy chickens in Spain. J Clin Microbiol. 1997. 35:2184–2185.

7. Blickwede M, Schwarz S. Molecular analysis of florfenicol-resistant Escherichia coli isolates from pigs. J Antimicrob Chemother. 2004. 53:58–64.

8. Bolton LF, Kelley LC, Lee MD, Fedorka-Cray PJ, Maurer JJ. Detection of multidrug-resistant Salmonella enterica serotype typhimurium DT104 based on a gene which confers cross-resistance to florfenicol and chloramphenicol. J Clin Microbiol. 1999. 37:1348–1351.

9. Boyd D, Cloeckaert A, Chaslus-Dancla E, Mulvey MR. Characterization of variant Salmonella genomic island 1 multidrug resistance regions from serovars Typhimurium DT104 and Agona. Antimicrob Agents Chemother. 2002. 46:1714–1722.

10. Briggs CE, Fratamico PM. Molecular characterization of an antibiotic resistance gene cluster of Salmonella typhimurium DT104. Antimicrob Agents Chemother. 1999. 43:846–849.

11. Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard. 2002. 2nd ed. Wayne: Clinical and Laboratory Standards Institute (formly NCCLs);CLSI M31-A2.

12. Cloeckaert A, Baucheron S, Chaslus-Dancla E. Nonenzymatic chloramphenicol resistance mediated by IncC plasmid R55 is encoded by a floR gene variant. Antimicrob Agents Chemother. 2001. 45:2381–2382.

13. Cloeckaert A, Baucheron S, Flaujac G, Schwarz S, Kehrenberg C, Martel JL, Chaslus-Dancla E. Plasmid-mediated florfenicol resistance encoded by floR gene in Escherichia coli isolated from cattle. Antimicrob Agents Chemother. 2000. 44:2858–2860.

14. Cloeckaert A, Sidi Boumedine K, Flaujac G, Imberechts H, D'Hooghe I, Chaslus-Dancla E. Occurrence of a Salmonella enterica serovar typhimurium DT104-like antibiotic resistance gene cluster including the floR gene in S. enterica serovar agona. Antimicrob Agents Chemother. 2000. 44:1359–1361.

15. Doublet B, Schwarz S, Nussbeck E, Baucheron S, Martel JL, Chaslus-Dancla E, Cloeckaert A. Molecular analysis of chromosomally florfenicol-resistant Escherichia coli isolates from France and Germany. J Antimicrob Chemother. 2002. 49:49–54.

16. Du X, Xia C, Shen J, Wu B, Shen Z. Characterization of florfenicol resistance among calf pathogenic Escherichia coli. FEMS Microbiol Lett. 2004. 236:183–189.

17. Hochhut B, Lotfi Y, Mazel D, Faruque SM, Woodgate R, Waldor MK. Molecular analysis of antibiotic resistance gene clusters in vibrio cholerae O139 and O1 SXT constins. Antimicrob Agents Chemother. 2001. 45:2991–3000.

18. Kehrenberg C, Schwarz S. Plasmid-borne florfenicol resistance in Pasteurella multocida. J Antimicrob Chemother. 2005. 55:773–775.

19. Keyes K, Hudson C, Maurer JJ, Thayer S, White DG, Lee MD. Detection of florfenicol resistance genes in Escherichia coli isolated from diseased chickens. Antimicrob Agents Chemother. 2000. 44:421–424.

20. Khan AA, Nawaz MS, Khan SA, Cerniglia CE. Detection of multidrug-resistant Salmonella typhimurium DT104 by multiplex polymerase chain reaction. FEMS Microbiol Lett. 2000. 182:355–360.

21. Kim E, Aoki T. Sequence analysis of the florfenicol resistance gene encoded in the transferable R-plasmid of a fish pathogen, Pasteurella piscicida. Microbiol Immunol. 1996. 40:665–669.

22. Shen J, Hu D, Wu X, Coats JR. Bioavailability and pharmacokinetics of florfenicol in broiler chickens. J Vet Pharmacol Ther. 2003. 26:337–341.

23. Singer RS, Patterson SK, Meier AE, Gibson JK, Lee HL, Maddox CW. Relationship between phenotypic and genotypic florfenicol resistance in Escherichia coli. Antimicrob Agents Chemother. 2004. 48:4047–4049.

24. Vassort-Bruneau C, Lesage-Descauses MC, Martel JL, Lafont JP, Chaslus-Dancla E. CAT III chloramphenicol resistance in Pasteurella haemolytica and Pasteurella multocida isolated from calves. J Antimicrob Chemother. 1996. 38:205–213.

25. White DG, Piddock LJ, Maurer JJ, Zhao S, Ricci V, Thayer SG. Characterization of fluoroquinolone resistance among veterinary isolates of avian Escherichia coli. Antimicrob Agents Chemother. 2000. 44:2897–2899.

26. White DG, Hudson C, Maurer JJ, Ayers S, Zhao S, Lee MD, Bolton L, Foley T, Sherwood J. Characterization of chloramphenicol and florfenicol resistance in Escherichia coli associated with bovine diarrhea. J Clin Microbiol. 2000. 38:4593–4598.

27. Yang H, Chen S, White DG, Zhao S, McDermott P, Walker R, Meng J. Characterization of multiple-antimicrobial-resistant Escherichia coli isolates from diseased chickens and swine in China. J Clin Microbiol. 2004. 42:3483–3489.