J Vet Sci.  2005 Dec;6(4):349-352.

Comparison of an enzyme-linked immunosorbent assay with serum neutralization test for serodiagnosis of porcine epidemic diarrhea virus infection

• Oh JS
• Song DS
• Yang JS
• Song JY
• Moon HJ
• Kim TY
• Park BK

Abstract

An indirect porcine epidemic diarrhea (PED) virus (PEDV) enzyme-linked immunosorbent assay (ELISA) was compared with the serum neutralization (SN) test by testing 46 samples from experimentally infected sows, 73 samples from naive sows, and 1, 024 field sow samples from 48 commercial swine farms of undefined PED status. The SN test and the ELISA were performed using PEDV, KPEDV-9 strain. Viral proteins as a coating antigen of PEDV ELISA were extracted from the cytoplasm of PEDV-infected Vero cells using a non-ionic detergent, Triton X-100, and a simple protocol of PEDV ELISA was followed. The presence of antibodies in these experimental samples was confirmed by SN and ELISA in which the sensitivity of the ELISA was 89.1%, and the corresponding specificity was 94.5%. On testing 1, 024 field samples, an overall agreement of 84.2% was generated between the SN and ELISA. This study demonstrates that the PEDV ELISA is a useful serodiagnostic screening test at herd level for detecting swine antibodies against PEDV.

Keyword

ELISA; porcine epidemic diarrhea virus; serum neutralization test

MeSH Terms

Animals
Antibodies, Viral/blood
Coronavirus Infections/diagnosis/*veterinary/virology
Diarrhea/diagnosis/*veterinary
Enzyme-Linked Immunosorbent Assay/veterinary
Female
Neutralization Tests/veterinary
Sensitivity and Specificity
Swine
Swine Diseases/diagnosis/*virology