Korean J Vet Res.  2016 Sep;56(3):189-192. 10.14405/kjvr.2016.56.3.189.

Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency

  • 1Veterinary Medicine and Biologics Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea. paru33@korea.kr
  • 2Avian Diseases Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.


The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The r² values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 log₁₀ and HI titer of 5 logâ‚‚ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.


Massachusetts 41 vaccine; hemagglutination inhibition; infectious bronchitis; potency test

MeSH Terms

Enzyme-Linked Immunosorbent Assay*
Hemagglutination Inhibition Tests*
Infectious bronchitis virus*
Neutralization Tests*
Vaccine Potency*
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