Korean J Vet Res.
2016 Sep;56(3):189-192. 10.14405/kjvr.2016.56.3.189.
Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency
- Affiliations
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- 1Veterinary Medicine and Biologics Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea. paru33@korea.kr
- 2Avian Diseases Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
- KMID: 2368766
- DOI: http://doi.org/10.14405/kjvr.2016.56.3.189
Abstract
- The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The r² values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 logâ‚â‚€ and HI titer of 5 logâ‚‚ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.
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