J Vet Sci.  2006 Dec;7(4):375-379. 10.4142/jvs.2006.7.4.375.

Attenuation of ischemia-reperfusion injury by ascorbic acid in the canine renal transplantation

• Lee JI
• Son HY
• Kim MC

• KMID: 1089486
• DOI: http://doi.org/10.4142/jvs.2006.7.4.375

Abstract

This study examined the effects of ascorbic acid on the attenuation of an ischemia-reperfusion (I/R) injury after a canine renal transplantation. Eight beagle dogs were subjected to a renal auto-transplantation followed by the administration of ascorbic acid (treatment group) and the same amount of vehicle (physiological saline, control group). Blood samples were collected from these dogs to perform the kidney function tests and the invasive blood pressure was measured in the renal artery at pre- and post-anastomosis. The antioxidant enzymes of level 72 h after the transplant were measured. The kidneys were taken for a histopathology evaluation at day 21. The kidney function tests showed a significant difference between the control and treatment group. The invasive blood pressure in the renal artery was similar in the groups. The activity of the antioxidant enzymes in the blood plasma was significant lower in the control group than in the treatment group. The histopathology findings revealed the treatment group to have less damage than the control group. The results of this study suggest that ascorbic acid alone might play a role in attenuating I/R injury and assist in the recovery of the renal function in a renal transplantation model.

Keyword

antioxidant; ascorbic acid; canine; renal transplantation

MeSH Terms

Animals
Ascorbic Acid/*therapeutic use
Blood Pressure
Blood Urea Nitrogen
Catalase/blood
Creatinine/blood
Dog Diseases/blood/*drug therapy/pathology
Dogs/*surgery
Female
Free Radical Scavengers/therapeutic use
Glutathione Peroxidase/blood
Histocytochemistry/veterinary
Kidney Transplantation/pathology/*veterinary
Male
Random Allocation
Reperfusion Injury/blood/drug therapy/pathology/*veterinary
Superoxide Dismutase/blood

Figure

• 

  Fig. 1 The total plasma SOD activity after renal auto transplantation followed by 72 h of reperfusion. The values are expressed as the mean ± SD for both groups. *p < 0.05; statistical significances between the control and treatment group.

• 

  Fig. 2 The plasma GSHPx activity after renal autotransplantation followed by 72 h of reperfusion. The values are expressed as the mean ± SD for both groups. *p < 0.05; statistical significances for control versus treatment group.

• 

  Fig. 3 The activity of plasma CAT after renal autotransplantation followed by 72 h of reperfusion. The values are expressed as mean ± SD for both groups. *p < 0.05; statistical significances between the control and treatment group.

• 

  Fig. 4 Microphotographs of the control group (A, B) and ascorbic acid treatment group (C, D). A; Showing partial loss of the lining cells and cystic dilation of the tubules. B; Medullar tubules showed focal apical or loss of apical cytoplasm. C; Showing mild regeneration of the tubular system. D; Tubular system of the medulla showing a normal cellular integrity. H&E stain, A, B, C; ×200, D; ×100.

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