Korean J Parasitol.  2003 Jun;41(2):129-133. 10.3347/kjp.2003.41.2.129.

Cloning of a pore-forming subunit of ATP-sensitive potassium channel from Clonorchis sinensis

Affiliations
  • 1Department of Parasitology and Institute of Malariology, Inje University College of Medicine, Busan 614-735, Korea.
  • 2Department of Microbiology, Inje University College of Medicine, Busan 614-735, Korea.
  • 3Department of Physiology and Biophysics, Inje University College of Medicine, Busan 614-735, Korea.
  • 4Molecular Cell Physiology Research Group, Inje University College of Medicine, Busan 614-735, Korea.

Abstract

A complete cDNA sequence encoding a pore-forming subunit (Kir6.2) of ATP-senstive potassium channel in the adult worm, Clonorchis sinensis, termed CsKir6.2, was isolated from an adult cDNA library. The cDNA contained a single open-reading frame of 333 amino acids, which has a structural motif (a GFG-motif) of the putative pore-forming loop of the Kir6.2. Peculiarly, the CsKir6.2 shows a lack-sequence structure, which deleted 57 amino acids were deleted from its N-terminus. The predicted amino acid sequence revealed a highly conserved sequence as other known other Kir6.2 subunits. The mRNA was weekly expressed in the adult worm.

Keyword

Clonorchis sinensis; potassium channels; cloning
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