Korean J Parasitol.  2003 Jun;41(2):129-133. 10.3347/kjp.2003.41.2.129.

Cloning of a pore-forming subunit of ATP-sensitive potassium channel from Clonorchis sinensis

• Hwang SY
• Han HJ
• Kim SH
• Park SG
• Seog DH
• Kim NR
• Han J
• Chung JY
• Kho WG

• KMID: 1080967
• DOI: http://doi.org/10.3347/kjp.2003.41.2.129

Abstract

A complete cDNA sequence encoding a pore-forming subunit (Kir6.2) of ATP-senstive potassium channel in the adult worm, Clonorchis sinensis, termed CsKir6.2, was isolated from an adult cDNA library. The cDNA contained a single open-reading frame of 333 amino acids, which has a structural motif (a GFG-motif) of the putative pore-forming loop of the Kir6.2. Peculiarly, the CsKir6.2 shows a lack-sequence structure, which deleted 57 amino acids were deleted from its N-terminus. The predicted amino acid sequence revealed a highly conserved sequence as other known other Kir6.2 subunits. The mRNA was weekly expressed in the adult worm.

Keyword

Clonorchis sinensis; potassium channels; cloning

MeSH Terms

Adenosine Triphosphate/metabolism
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Clonorchis sinensis/*genetics/metabolism
Helminth Proteins/*genetics/metabolism
Human
Molecular Sequence Data
Potassium Channels, Inwardly Rectifying/*genetics/metabolism
RNA, Helminth/chemistry/genetics
Sequence Alignment
Support, Non-U.S. Gov't