Korean J Parasitol.  2000 Dec;38(4):257-261. 10.3347/kjp.2000.38.4.257.

The effect of microfilament inhibitor on the Cryptosporidium infection in vitro

Affiliations
  • 1Department of Parasitology, College of Medicine, Konkuk University, Chungju 380-701, Korea. jaeran.yu@mail.kku.ac.kr

Abstract

This study was focused on the effects of microfilament inhibitor, Cytochalasin D (CD) on the invasiveness of sporozoites of Cryptosporidium spp. into the host cells. MDCK and AGS cell lines were used as host cells for C. parvum and C. muris, respectively. When MDCK cells were pretreated with CD for 1 hr before inoculation of the sporozoites, C. parvum infection was significantly inhibited when compared to the control cells. These inhibitory effects of CD on the rate of infection were dose-dependent. In addition, C. muris infection was hampered when AGS cell lines were pretreated with CD. However, the capability of invasiveness of the sporozoites into the host cells was not greatly influenced by the pretreatment of sporozoites with CD before infection. These results suggest that microfilaments of host cells, rather than parasites, play an important role for the invasion of Cryptosporidium spp.


MeSH Terms

Animal
Cells, Cultured
Cryptosporidiosis/etiology
Cryptosporidiosis/drug therapy*
Cryptosporidium/pathogenicity
Cytochalasin D/therapeutic use*
Cytochalasin D/pharmacology
Dogs
Dose-Response Relationship, Drug
Human
Microfilaments/physiology
Microfilaments/drug effects*
Full Text Links
  • KJP
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr