Exp Mol Med.  2008 Apr;40(2):229-236. 10.3858/emm.2008.40.2.229.

Implication of leucyl-tRNA synthetase 1 (LARS1) over-expression in growth and migration of lung cancer cells detected by siRNA targeted knock-down analysis

Affiliations
  • 1Department of Microbiology, The Catholic University of Korea, Seoul 137-701, Korea. yejun@catholic.ac.kr
  • 2Integrated Research Center for Genome Polymorphism,The Catholic University of Korea, Seoul 137-701, Korea.
  • 3Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.

Abstract

Molecular mechanism of lung carcinogenesis and its aggressive nature is still largely elusive. To uncover the biomarkers related with tumorigenesis and behavior of lung cancer, we screened novel differentially expressed genes (DEG) in A549 lung cancer cell line by comparison with CCD-25Lu, normal pulmonary epithelial cell line, using annealing control primer(ACP)- based GeneFishing system. Of the DEGs, over-expression of leucyl-tRNA synthetase 1 (LARS1) was prominent and this up-regulation was confirmed by immunoblotting and real-time quantitative RT-PCR analysis. In addition to A549 cell line, primary lung cancer tissues also expressed higher level of LARS1 mRNA than their normal counter tissues. To explore the oncogenic potential of LARS1 over-expression in lung cancer, we knocked-down LARS1 by treating siRNA and observed the tumor behavior. LARS1 knock-down cells showed reduced ability to migrate through transwell membrane and to form colonies in both soft agar and culture plate. Taken together, these findings suggest that LARS1 may play roles in migration and growth of lung cancer cells, which suggest its potential implication in lung tumorigenesis.

Keyword

amino acyl-tRNA synthetases; cell movement; cell proliferation; leucine-tRNA ligase; lung neoplasms; oncogenes; RNA, small interfering
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