J Korean Med Sci.  2000 Aug;15(Suppl):S42-S43. 10.3346/jkms.2000.15.S.S42.

Beta agonist regulation of sodium transport in fetal lung epithelium: roles of cell volume, cytosolic chloride and protein tyrosine kinase

• Marunaka Y
• Niisato N
• Ito Y

• KMID: 1026947
• DOI: http://doi.org/10.3346/jkms.2000.15.S.S42

Abstract

1) A beta agonist stimulated Na+ transport and decreased the intracellular Cl concentration ([Cl]c) associated with cell shrinkage via an increase in cytosolic cAMP level by activating adenylate cyclase in rat fetal distal lung epithelial (FDLE) cells. 2) Lowering [Cl-]c activated a 28-pS nonselective cation (NSC) channel by elongating the open time of the channel. 3) cAMP signals were converted to a protein tyrosine kinase (PTK)-mediated signal. 4) The PTK-mediated signal was involved in the cAMP-stimulated Na+ transport in rat FDLE cells.

Keyword

cAMP; Tyrosine Phosphorylation; Protein Kinase A; Protein Tyrosine Kinase; Alveolar Epithelium

MeSH Terms

Adrenergic beta-Agonists/pharmacology*
Animal
Biological Transport/physiology
Biological Transport/drug effects
Cell Size/physiology
Chlorides/metabolism*
Cyclic AMP/metabolism
Cytosol/metabolism
Enzyme Inhibitors/pharmacology
Female
Fetus/cytology
Forskolin/pharmacology
Nitrobenzoates/pharmacology
Pregnancy
Protein-Tyrosine Kinase/metabolism*
Rats
Rats, Wistar
Respiratory Mucosa/enzymology*
Respiratory Mucosa/embryology
Respiratory Mucosa/cytology
Sodium/metabolism*
Tyrphostins/pharmacology