Exp Mol Med.
2011 Nov;43(11):622-629. 10.3858/emm.2011.43.11.070.
Enhanced skin wound healing by a sustained release of growth factors contained in platelet-rich plasma
- Affiliations
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- 1School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea. byungskim@snu.ac.kr
- 2Department of Orthopaedics, Dongguk University International Hospital, Goyang 411-773, Korea.
- 3Department of Periodontology, College of Dentistry, Yonsei University, Seoul 120-752, Korea.
- KMID: 1025165
- DOI: http://doi.org/10.3858/emm.2011.43.11.070
Abstract
- Platelet-rich plasma (PRP) contains growth factors that promote tissue regeneration. Previously, we showed that heparin-conjugated fibrin (HCF) exerts the sustained release of growth factors with affinity for heparin. Here, we hypothesize that treatment of skin wound with a mixture of PRP and HCF exerts sustained release of several growth factors contained in PRP and promotes skin wound healing. The release of fibroblast growth factor 2, platelet-derived growth factor-BB, and vascular endothelial growth factor contained in PRP from HCF was sustained for a longer period than those from PRP, calcium-activated PRP (C-PRP), or a mixture of fibrin and PRP (F-PRP). Treatment of full-thickness skin wounds in mice with HCF-PRP resulted in much faster wound closure as well as dermal and epidermal regeneration at day 12 compared to treatment with either C-PRP or F-PRP. Enhanced skin regeneration observed in HCF-PRP group may have been at least partially due to enhanced angiogenesis in the wound beds. Therefore, this method could be useful for skin wound treatment.
Keyword
MeSH Terms
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Animals
Blotting, Western
*Cell Proliferation
Dermis/cytology/metabolism
Female
Fibrin/*metabolism
Fibroblast Growth Factor 2/genetics/metabolism
Heparin/metabolism
Immunoenzyme Techniques
Intercellular Signaling Peptides and Proteins/*secretion
Mice
Mice, Inbred BALB C
Platelet-Rich Plasma/*metabolism
Proto-Oncogene Proteins c-sis/genetics/metabolism
RNA, Messenger/genetics
Real-Time Polymerase Chain Reaction
Regeneration
Skin/*cytology/*metabolism
Vascular Endothelial Growth Factor A/genetics/metabolism
Wound Healing/*physiology
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