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Phylogenetic Analysis and Rapid Detection of Genus Phellinus using the Nucleotide Sequences of 18S Ribosomal RNA

Nam BH, Lee JY, Kim GY, Jung HH, Park HS, Kim CY, Jo WS, Jeong SJ, Lee TH, Lee JD

Analysis of phylogenetic relationship was performed among Phellinus species based on 18S ribosomal subunit sequence data. Twenty-five strains of 19 Phellinus species including P. linteus were examined in this study....
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Identification of Cystoisospora ohioensis in a Diarrheal Dog in Korea

Lee S, Kim J, Cheon DS, Moon EA, Seo DJ, Jung S, Shin H, Choi C

A 3-month-old female Maltese puppy was hospitalized with persistent diarrhea in a local veterinary clinic. Blood chemistry and hematology profile were analyzed and fecal smear was examined. Diarrheal stools were...
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Variation of nuclear and mitochondrial DNAs in Korean and Chinese isolates of Clonorchis sinensis

Lee SU, Huh S

We compared the DNA sequence difference of isolates of Clonorchis sinensis from one Korean (Kimhae) and two Chinese areas (Guangxi and Shenyang). The sequences of nuclear rDNA (18S, internal transcribed...
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Genetic Characterization of Clinical Acanthamoeba Isolates from Japan using Nuclear and Mitochondrial Small Subunit Ribosomal RNA

Rahman MM, Yagita K, Kobayashi , Oikawa Y, Hussein AI, Matsumura , Tokoro M

Because of an increased number of Acanthamoeba keratitis (AK) along with associated disease burdens, medical professionals have become more aware of this pathogen in recent years. In this study, by...
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A Case of Trichosporon inkin Continuous Ambulatory Peritoneal Dialysis Peritonitis Identified by 18S rRNA Sequencing

Lee HS, Choi JH, Kim SM, Shim YK, Seong MH, Kim HY, Shin KS, Kwon SK

  • KMID: 2252653
  • Korean J Nephrol.
  • 2011 Sep;30(5):561-564.
Fungal peritonitis in peritoneal dialysis patients is hard to treat without catheter removal and shows higher mortality. Although Candida species is the most common pathogen of fungal peritonitis, there are...
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Identification of parasite DNA in common bile duct stones by PCR and DNA sequencing

Jang JS, Kim KH, Yu JR, Lee SU

We attempted to identify parasite DNA in the biliary stones of humans via PCR and DNA sequencing. Genomic DNA was isolated from each of 15 common bile duct (CBD) stones...
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Ancient Mitochondrial DNA Analyses of Ascaris Eggs Discovered in Coprolites from Joseon Tomb

Oh CS, Seo M, Hong JH, Chai JY, Oh SW, Park JB, Shin DH

Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in...
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Subgenus classification of Acanthamoeba by riboprinting

Chung DI, Yu HS, Hwang MY, Kim TH, Kim TO, Yun HC, Kong HH

Subgenus classification of Acanthamoeba remains uncertain. Twenty-three reference strains of Acanthamoeba including 18 (neo)type-strains were subjected for classification at the subgenus level by riboprinting. PCR/RFLP analysis of 18S rRNA gene...
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Molecular Description of Macroorchis spinulosus (Digenea: Nanophyetidae) Based on ITS1 Sequences

Won EJ, Kim DG, Cho J, Jung BK, Kim MJ, Yun YW, Chai JY, Ryang DW

We performed a molecular genetic study on the sequences of 18S ribosomal RNA (ITS1 region) gene in 4-day-old adult worms of Macroorchis spinulosus recovered in mice experimentally infected with metacercariae...
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Multiplex-Touchdown PCR to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the Major Causes of Traveler's Diarrhea

Shin JH, Lee SE, Kim TS, Ma DW, Chai JY, Shin EH

This study aimed to develop a multiplex-touchdown PCR method to simultaneously detect 3 species of protozoan parasites, i.e., Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the major causes of traveler’s...
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Occurrence and Molecular Identification of Giardia duodenalis from Stray Cats in Guangzhou, Southern China

Zheng G, Hu W, Liu Y, Luo Q, Tan L, Li G

The objective of this study was to genetically characterize isolates of Giardia duodenalis and to determine if zoonotic potential of G. duodenalis could be found in stray cats from urban...
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A Case of Plasmodium ovale Malaria Imported from West Africa

Kang Y, Yang J

Malaria is a parasitic infection caused by Plasmodium species. Most of the imported malaria in Korea are due to Plasmodium vivax and Plasmodium falciparum, and Plasmodium ovale infections are very...
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Evaluation of taxonomic validity of four species of Acanthamoeba: A. divionensis, A. paradivionensis, A. mauritaniensis, and A. rhysodes, inferred from molecular analyses

Liu H, Moon EK, Yu HS, Jeong HJ, Hong YC, Kong HH, Chung DI

The taxonomy of Acanthamoeba spp., an amphizoic amoeba which causes granulomatous amoebic encephalitis and chronic amoebic keratitis, has been revised many times. The taxonomic validity of some species has yet...
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Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates

Xuan YH, Chung BS, Hong YC, Kong HH, Hahn TW, Chung DI

Three Acanthamoeba isolates (KA/E9, KA/E17, and KA/E23) from patients with keratitis were identified as Acanthamoeba triangularis by analysis of their molecular characteristics, a species not previously recognized to be a...
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Molecular Characterization of Various Trichomonad Species Isolated from Humans and Related Mammals in Indonesia

Kamaruddin M, Tokoro M, Rahman MM, Arayama S, Hidayati A, Syafruddin D, Asih PB, Yoshikawa , Kawahara E

Trichomonad species inhabit a variety of vertebrate hosts; however, their potential zoonotic transmission has not been clearly addressed, especially with regard to human infection. Twenty-one strains of trichomonads isolated from...
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Genotyping of Giardia duodenalis Isolates from Dogs in Guangdong, China Based on Multi-Locus Sequence

Zheng G, Alsarakibi M, Liu Y, Hu W, Luo Q, Tan L, Li G

  • KMID: 1792186
  • Korean J Parasitol.
  • 2014 Jun;52(3):299-304.
This study aimed to identify the assemblages (or subassemblages) of Giardia duodenalis by using normal or nested PCR based on 4 genetic loci: glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi),...
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Molecular Differentiation of Schistosoma japonicum and Schistosoma mekongi by Real-Time PCR with High Resolution Melting Analysis

Kongklieng A, Kaewkong W, Intapan PM, Sanpool O, Janwan P, Thanchomnang T, Lulitanond V, Sri-Aroon , Limpanont , Maleewong

Human schistosomiasis caused by Schistosoma japonicum and Schistosoma mekongi is a chronic and debilitating helminthic disease still prevalent in several countries of Asia. Due to morphological similarities of cercariae and...
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Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Acanthamoeba

Yang HW, Lee YR, Inoue N, Jha BK, Danne DB, Kim HK, Lee J, Goo YK, Kong HH, Chung DI, Hong Y

Amoebic keratitis (AK) caused by Acanthamoeba is one of the most serious corneal infections. AK is frequently misdiagnosed initially as viral, bacterial, or fungal keratitis, thus ensuring treatment delays. Accordingly,...
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PCR Detection and Molecular Characterization of Pentatrichomonas hominis from Feces of Dogs with Diarrhea in the Republic of Korea

Kim YA, Kim HY, Cho SH, Cheun HI, Yu JR, Lee SE

Pentatrichomonas hominis is considered a commensal protozoan in the large intestine of a number of mammalian hosts, such as cats, dogs, and non-human primates. The resulting infections, which can induce...
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Urogonimus turdi (Digenea: Leucochloridiidae) from the White’s Thrush, Zoothera aurea, in the Republic of Korea

Kim HC, Hong EJ, Ryu SY, Park J, Yu DH, Chae JS, Choi KS, Sim C, Park BK

Avian trematodes, Urogonimus turdi (Digenea: Leucochloridiidae), were collected from the intestine of wild birds, Zoothera aurea, 2013–2017 in the Daejeon Metropolitan City, Korea. The body was ellipsoidal, attenuated and/or round...
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