Korean J Parasitol.  2014 Jun;52(3):299-304.

Genotyping of Giardia duodenalis Isolates from Dogs in Guangdong, China Based on Multi-Locus Sequence

• Zheng G
• Alsarakibi M
• Liu Y
• Hu W
• Luo Q
• Tan L
• Li G

Abstract

This study aimed to identify the assemblages (or subassemblages) of Giardia duodenalis by using normal or nested PCR based on 4 genetic loci: glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), beta-giardin (bg), and small subunit ribosomal DNA (18S rRNA) genes. For this work, a total of 216 dogs' fecal samples were collected in Guangdong, China. The phylogenetic trees were constructed with MEGA5.2 by using the neighbor-joining method. Results showed that 9.7% (21/216) samples were found to be positive; moreover, 10 samples were single infection (7 isolates assemblage A, 2 isolates assemblage C, and 1 isolate assemblage D) and 11 samples were mixed infections where assemblage A was predominant, which was potentially zoonotic. These findings showed that most of the dogs in Guangdong were infected or mixed-infected with assemblage A, and multi-locus sequence typing could be the best selection for the genotype analysis of dog-derived Giardia isolates.

Keyword

Giardia duodenalis; dog; multi-locus sequence typing; Guangdong; China

MeSH Terms

Animals
China
Cluster Analysis
Coinfection/parasitology/veterinary
Cytoskeletal Proteins/genetics
DNA, Protozoan/chemistry/genetics
Dog Diseases/parasitology
Dogs
Genotype
Giardia lamblia/*classification/*genetics/isolation & purification
Giardiasis/parasitology/*veterinary
Glutamate Dehydrogenase/genetics
Molecular Sequence Data
*Multilocus Sequence Typing
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 18S/genetics
Triose-Phosphate Isomerase/genetics
Cytoskeletal Proteins
DNA, Protozoan
Glutamate Dehydrogenase
RNA, Ribosomal, 18S
Triose-Phosphate Isomerase