Korean J Parasitol.  2004 Sep;42(3):145-148. 10.3347/kjp.2004.42.3.145.

Variation of nuclear and mitochondrial DNAs in Korean and Chinese isolates of Clonorchis sinensis

• Lee SU
• Huh S

• KMID: 662786
• DOI: http://doi.org/10.3347/kjp.2004.42.3.145

Abstract

We compared the DNA sequence difference of isolates of Clonorchis sinensis from one Korean (Kimhae) and two Chinese areas (Guangxi and Shenyang). The sequences of nuclear rDNA (18S, internal transcribed spacer 1 and 2: ITS1 and ITS2) and mitochondrial DNA (cytochrome c oxidase subunit 1: cox1) were compared. A very few intraspecific nucleotide substitution of the 18S, ITS1, ITS2 and cox1 was found among three isolates of C. sinensis and a few nucleotide insertion and deletion of ITS1 were detected. The 18S, ITS1, ITS2 and cox1 sequences were highly conserved among three isolates. These findings indicated that the Korean and two Chinese isolates are similar at the DNA sequence level.

Keyword

Clonorchis sinensis; ribosomal DNA; mitochondrial DNA; China; Korea

MeSH Terms

Animals
Base Sequence
China
Clonorchis sinensis/enzymology/*genetics
Comparative Study
DNA, Helminth/chemistry/*genetics
DNA, Mitochondrial/chemistry/*genetics
DNA, Ribosomal/chemistry/genetics
DNA, Ribosomal Spacer/chemistry
Electron Transport Complex IV/genetics
Genetic Markers
Korea
Molecular Sequence Data
RNA, Ribosomal, 18S/genetics
Research Support, Non-U.S. Gov't
Sequence Alignment
Species Specificity
*Variation (Genetics)