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An Effective Method of RNA Extraction from Mycobacterium tuberculosis

Oh TS, Kang HY, Nam YS, Kim YJ, You EK, Lee MY, Cho SY, Lee HJ

In the RNA-based study, it is important to extract high-quality RNA. However, RNA extraction from Mycobacterium tuberculosis is problematic due to its thick, waxy cell wall rich in mycolic acid,...
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Mycolic Acid Analysis for Identification of Mycobacterium spp. using High-Performance Liquid Chromatography

Lee HW, Cho BW, Kang MS, Kim JS, Lee JW, Nahm CH, Choi JW, Kim JJ, Pai SH

  • KMID: 1863294
  • Korean J Clin Pathol.
  • 1998 Mar;18(1):77-84.
BACKGROUND: Mycobacteria are traditionally identified with biochemical reactions. Since it takes 2 to 6 weeks, more rapid method is needed for timely treatment of mycobacterial infection. Mycolic acid analysis by...
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Comparison of Mycobacterial Culture by Mycobacterium Growth Indicator Tube and Ogawa Media

Joung US, Jeong J, Lee SH, Kim SR

  • KMID: 1892728
  • Korean J Clin Microbiol.
  • 2004 Sep;7(2):135-138.
BACKGROUND: Mycobacterial disease is still greatly concerned in the developing and industrialized countries. Ogawa media has been used to diagnose mycobacterial disease in Korea in spite of a low sensitivity...
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Distribution and Clinical Significance of Nontuberculous Mycobacteria Identified by High Performance Liquid Chromatography in Clinical Specimens

Jeong J, Kim SR, Chang CL, Lee SH

BACKGROUND: Infections caused by nontuberculous mycobacteria (NTM) are significantly increasing over the last decade. Due to the uncertainty in the clinical significance of these organisms, their effective diagnosis and treatment...
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Acid-Fastness of Histoplasma in Surgical Pathology Practice

Rajeshwari M, Xess I, Sharma MC, Jain D

BACKGROUND: Histoplasmosis (HP) is diagnosed by visualizing intracellular microorganisms in biopsy and/or culture. Periodic-acid Schiff (PAS) and Gomori methenamine silver (GMS) staining methods are routinely used for identification. The acid-fast...
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A Novel Synthetic Mycolic Acid Inhibits Bronchial Hyperresponsiveness and Allergic Inflammation in a Mouse Model of Asthma

Kim YJ, Kim HJ, Jeong SK, Lee SH, Kang MJ, Yu HS, Jung YH, Seo JH, Kim BJ, Yu J, Park SJ, Lee YC, Hong SJ

PURPOSE: Recognition of microbes is important to trigger the innate immune system. Mycolic acid (MA) is a component of the cell walls of mycobacteria such as Mycobacterium bovis Bacillus Calmette-Guerin....
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Identification of Mycobacteria Species by HPLC and Species Distribution during Five Years at Ulsan University Hospital

Jeong J, Kim SR, Chang CL, Lee SH

BACKGROUND: Infections caused by mycobacteria have been significantly increasing. Due to the difficulty of making a decision about the pathogenicity of mycobacteria, species-level identification is very important for patients' diagnosis...
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Lung Infection Caused by Mycobacterium riyadhense Confused with Mycobacterium tuberculosis: The First Case in Korea

Choi JI, Lim JH, Kim SR, Lee SH, Park JS, Seo KW, Jeon JB, Jeong J

A slowly growing, non-chromogenic mycobacterial strain was isolated from sputum and bronchial lavage fluid samples of a patient presenting with productive cough, blood-tinged sputum, low-grade fever, and weakness. A positive...
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