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Essential Elements for Establishing Clinical Next-generation Sequencing Testing

Park KJ, Lee W, Chun S, Min WK

Over the past decade, next-generation sequencing (NGS) has evolved at an astonishing pace and has revolutionized clinical medicine as well as genomics research. The rapid advancements in NGS technologies have...
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A Case of Urinary Tract Infection Caused by the Emerging Uropathogen Actinotignum schaalii

Kwon WK, Song DJ, Huh HJ, Park SH, Ki CS, Lee NY

Actinotignum schaalii is an emerging uropathogen; however, routine culture protocols and usual phenotypic methods do not allow for easy detection and identification. Herein, we report the first Korean case of...
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A Case of Mature B-cell Neoplasm with Light Chain Clonality Confirmed by Cytoplasmic Light Chain Expression Using Flow Cytometry

Cho EH, Yoo IY, Ryu MR, Shin HY, Kim SH, Cho D

Surface immunoglobulin light-chain restriction is evidence of clonality in mature B-cell neoplasms. An aberrant pattern of surface light-chain expression can also be considered evidence of clonality. However, because this result...
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A Report of Bone Marrow Metastasis of Colon Cancer as a Primary Diagnosis, Supported by Cytokeratin Immunohistochemical Staining

Kim B, Lee KT

Bone marrow metastasis of colon cancer is rare. Here, we report a 56-year-old female patient who presented with pancytopenia. She was diagnosed with colon cancer accompanied by lung and axial...
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Performance Evaluation of Two Immunochromatographic Assays for Rotavirus Detection in Stool Specimens

Kim S, Kim YJ, Cho SY, Park TS, Lee HJ

BACKGROUND: Rotavirus is a major pathogen causing enteritis worldwide in children under five years of age. In recent years, immunochromatographic assay (ICA) has been widely used as a diagnostic test...
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A Case of Fungemia with Co-isolation of Candida parapsilosis and Trichosporon asahii Confirmed by MALDI-TOF MS and D2 rRNA Sequencing

Seo JD, Kim NH, Hong YJ, Kim TS, Hwang SM, Park JS, Seong MW, Park KU, Song J, Park SS, Kim EC

Fungi are a major cause of human infections with diverse clinical manifestations. The incidence of fungal infections has increased over time, particularly in patients who have risk factors such as...
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A Clue to Discovering Unstable Hemoglobin Variants via Abnormal WBC Differential Scattergrams Using the Sysmex Automated Hematology Analyzer

Park S, Jeong TD, Hong KS, Huh J

Incidentally, hemoglobin (Hb) variants can be detected using HbA1c tests in clinical laboratories. We found 38 patients with Hb variants after reviewing a total of 29,398 HbA1c test results from...
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Comparison between Medion Vacuon Tube and BD Vacutainer Tube for Clinical Laboratory Practice

Jhang J, Cho JY, Lee JH, Kim J

BACKGROUND: Most of the blood-test samples are collected and carried in vacuum tubes. We have compared a newly developed vacuum tube, ‘Vacuon’ (Medion, Korea) and ‘BD Vacutainer®’ (BD, USA) in...
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Two Cases of Hypersensitivity Reactions Caused by Human Serum Albumin During Therapeutic Plasma Exchange

Kim TY, Shin DW, Hong YJ, Kim H, Park KU, Han KS

Iso-oncotic human serum albumin (HSA) is the primary replacement fluid of choice during therapeutic plasma exchange (TPE). Hypersensitivity reactions to HSA are rare, but require proper evaluation and management. In...
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Evaluation of Time and Temperature Stability of Paroxysmal Nocturnal Hemoglobinuria Cells by Flow Cytometry

Lee SH, Bang HI, Shin YJ, Shin WY, Kim J, Park R, Shin JW, Choi TY

BACKGROUND: Flow cytometry analysis of paroxysmal nocturnal hemoglobinuria (PNH) is significantly affected by the methodology used. The lack of data on the effect of age and refrigeration on PNH clone...
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Effects of Pre-analytical Variables on Cell-free DNA Extraction for Liquid Biopsy

Jeong TD, Kim MH, Park S, Chung HS, Lee JW, Chang JH, Huh J

BACKGROUND: Extraction of cell-free DNA (cfDNA) is a key step for determining the quality of cfDNA-related molecular diagnostics. We evaluated the effect of sample containers and sample storage conditions on...
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Trends in Bloodstream Infections and Antimicrobial Susceptibilities at a University Hospital in Korea Between 2007 and 2016

Lim S, Yeom JS, Joo EJ, Cheong HS, Lee K, Woo HY, Park H, Kwon MJ

BACKGROUND: Blood culture is an important method for identifying infectious microorganisms and confirming that a selected antimicrobial treatment is appropriate. In this study, we investigated the annual changes in the...
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