- Viral vectors for vaccine applications
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Choi Y, Chang J
- KMID: 2167719
- Clin Exp Vaccine Res.
- 2013 Jul;2(2):97-105.
- doi: 10.7774/cevr.2013.2.2.97
Traditional approach of inactivated or live-attenuated vaccine immunization has resulted in impressive success in the reduction and control of infectious disease outbreaks. However, many pathogens remain less amenable to deal... -
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- An Efficient Method to Prepare PCR Cloning Vectors
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Hong SG, Choi JY, Pryor BM, Lee HK
- KMID: 2053021
- Mycobiology.
- 2009 Sep;37(3):240-242.
- doi: 10.4489/MYCO.2009.37.3.240
An improved procedure for preparing PCR cloning vectors was developed. This procedure includes the incorporation of adapters to create XcmI restriction enzyme sites in pBluescript II SK(+) vectors, digestion with... -
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- Construction of Various Copy Number Plasmid Vectors and Their Utility for Genome Sequencing
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Yang TJ, Yu Y, Frisch DA, Lee S, Kim HR, Kwon SJ, Park BS, Wing RA
- KMID: 1515374
- Genomics Inform.
- 2004 Dec;2(4):174-179.
We developed various plasmid cloning vectors that are useful in the construction of genomic and shotgun libraries. Two medium copy vectors, pCUGIblu21(pCb21) and pAGIblu21 (pAb21), which are resistant to kanamycin... -
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- Induction of humoral responses to BHV-1 glycoprotein D expressed by HSV-1 amplicon vectors
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Blanc AM, Berois MB, Tome LM, Epstein AL, Arbiza JR
- KMID: 1365004
- J Vet Sci.
- 2012 Mar;13(1):59-65.
- doi: 10.4142/jvs.2012.13.1.59
Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile and useful tools for transferring genes into cells that are capable of stimulating a specific immune response to their expressed antigens.... -
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- Distribution of Adenoviral Vector in Brain after Intravenous Administration
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Jin JY, Moon CI, Moon CI, Kang WS, Jeong DC
- KMID: 1099803
- J Korean Med Sci.
- 2003 Feb;18(1):108-111.
- doi: 10.3346/jkms.2003.18.1.108
The delivery of transgenes to the central nervous system (CNS) can be a valuable tool to treat CNS diseases. Various systems for the delivery to the CNS have been developed;... -
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- Construction of a Mycobacterium - Escherichia coli Shuttle Vector and Use in the Expression of Foreign Genes in Mycobacteria
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Cho SN, Kim JD, Lee HY, Kim HJ
- KMID: 1540524
- J Korean Soc Microbiol.
- 1997 Jun;32(3):293-300.
The ability to introduce recombinant DNA molecules back into mycobacteria would greatly increase the potential of molecular genetic approaches for the study of mycobacteria as well as for the use... -
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- Monitoring transfected cells without selection agents by using the dual-cassette expression EGFP vectors
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Kang SG, Lee EK, Schaus S, Henderson
- KMID: 938883
- Exp Mol Med.
- 2001 Sep;33(3):174-178.
Conventional methods of selecting gene transfected cells by toxic agents may yield ambiguous results. It is difficult to determine whether cell death is due to selection agents or gene transfection,... -
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- Promoter effects of adeno-associated viral vector for transgene expression in the cochlea in vivo
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Liu Y, Okada T, Nomoto T, Ke X, Kume A, Ozawa K, Xiao S
- KMID: 1094657
- Exp Mol Med.
- 2007 Apr;39(2):170-175.
The aims of this study were to evaluate the expression of enhanced green fluorescent protein (EGFP) driven by 6 different promoters, including cytomegalovirus IE enhancer and chicken beta-actin promoter (CAG),... -
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- Inhibition of in vitro hepatitis B virus replication by lentivirus-mediated short-hairpin RNA against HBx
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Kim JW, Lee SH, Park YS, Jeong SH, Kim N, Lee DH
- KMID: 1776811
- Korean J Hepatol.
- 2009 Mar;15(1):15-24.
- doi: 10.3350/kjhep.2009.15.1.15
BACKGROUNDS/AIMS: Hepatitis B virus (HBV) replicates via RNA intermediates, which could serve as targets for RNA interference (RNAi). Vector-mediated short-hairpin RNA (shRNA) can induce sustained RNAi in comparison to small... -
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- Development of porcine respiratory and reproductive syndrome virus replicon vector for foot-and-mouth disease vaccine
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Jeeva S, Lee JA, Park SY, Song CS, Choi IS, Lee JB
- KMID: 2049100
- Clin Exp Vaccine Res.
- 2014 Jan;3(1):100-109.
- doi: 10.7774/cevr.2014.3.1.100
PURPOSE: Foot-and-mouth disease (FMD) is an economically important global animal disease. To control FMD virus (FMDV) outbreaks, a lot of different novel approaches have been attempted. In this study, we... -
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- Low-dose radiation response of the p21(WAF1/CIP1) gene promoter transduced by adeno-associated virus vector
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Nenoi M, Daino K, Ichimura S, Takahash S, Akuta T
- KMID: 1098090
- Exp Mol Med.
- 2006 Oct;38(5):553-564.
In cancer gene therapy, restriction of antitumor transgene expression in a radiation field by use of ionizing radiation-inducible promoters is one of the promising approaches for tumor-specific gene delivery. Although... -
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- Expression of Hepatitis C Virus Core Protein in Hepatocytes Does Not Modulate Proliferation or Apoptosis of CD8+ T Cells
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Jin YH, Crispe IN, Park S
- KMID: 2428300
- Yonsei Med J.
- 2005 Dec;46(6):827-834.
Hepatocytes are the primary targets of the hepatitis C virus (HCV). While immunosuppressive roles of HCV core protein have been found in several studies, it remains uncertain whether core protein... -
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- Inhibition of hepatitis B virus replication by RNA interference
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Park YG
- KMID: 1776809
- Korean J Hepatol.
- 2009 Mar;15(1):1-6.
- doi: 10.3350/kjhep.2009.15.1.1
No abstract available. -
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- Cardiac expression profiles of the naked DNA vectors encoding vascular endothelial growth factor and basic fibroblast growth factor
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Lee JS, Byun J, Kim JM, Kim CY, Kim BM, Chung JH, Jang Y, Kim DK
- KMID: 1089454
- Exp Mol Med.
- 2005 Oct;37(5):447-456.
We investigated expression profiles and biological effects of the naked DNA vectors in the heart. To this end, naked DNA vector was injected into the apex of the beating rat... -
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- Delivery of Factor VIII Gene into Skeletal Muscle Cells Using Lentiviral Vector
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Jeon HJ, Oh TK, Kim OH, Kim ST
- KMID: 1779606
- Yonsei Med J.
- 2010 Jan;51(1):52-57.
- doi: 10.3349/ymj.2010.51.1.52
PURPOSE: This study was designed to investigate whether transduction of lentiviral vectors (LV) carrying human coagulation factor VIII (hFVIII) cDNA into skeletal muscle could increase circulating hFVIII concentrations. MATERIALS AND... -
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- Effects of adenovirus-mediated bFGF, IL-1Ra and IGF-1 gene transfer on human osteoarthritic chondrocytes and osteoarthritis in rabbits
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Chen B, Qin J, Wang H, Magdalou J, Chen L
- KMID: 964663
- Exp Mol Med.
- 2010 Oct;42(10):684-695.
- doi: 10.3858/emm.2010.42.10.067
The study investigated the effects of adenovirus-mediated gene transfection of basic fibroblast growth factor (bFGF), bFGF combined with interleukin-1 receptor antagonist protein (IL-Ra) and/or insulin-like growth factor-1 (IGF-1) both in... -
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- Attenuation of the Expression of Fibrogenic Molecules by Transforming Growth Factor-beta1 Antisense in Cultured Rat Mesangial Cells
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Shin GT, Kim H
- KMID: 1610168
- Korean J Nephrol.
- 2003 Jul;22(4):349-357.
BACKGROUND: The diabetic milieu augments the expression of transforming growth factor-beta1 (TGF-beta1) in mesangial cells, which, in turn, stimulates the accumulation of extracellular matrix in glomeruli. Accordingly, mesangial cells have... -
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- Organotypic slice culture of the hypothalamic paraventricular nucleus of rat
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Cho ES, Lee SY, Park JY, Hong SG, Ryu PD
- KMID: 1104231
- J Vet Sci.
- 2007 Mar;8(1):15-20.
- doi: 10.4142/jvs.2007.8.1.15
Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged... -
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- Prolonged hypotensive effect of human tissue kallikrein gene delivery and recombinant enzyme administration in spontaneous hypertension rats
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Sun H, Zhang L, Wang A, Xue Z
- KMID: 755661
- Exp Mol Med.
- 2004 Feb;36(1):23-27.
To evaluate the feasibility of treating hypertension by human tissue kallikrein gene (KLK1) delivery and by enzyme (rK1) administration, two recombinant vectors expressing KLK1 cDNA were constructed for gene delivery... -
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- p53 gene transfer does not enhance E2F-1-mediated apoptosis in human colon cancer cells
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Draus JM, Elliott MJ, Atienza C, Stilwell A, Wong SL, Dong Y, Yang H, McMasters
- KMID: 1457532
- Exp Mol Med.
- 2001 Dec;33(4):209-219.
E2F-1 and p53 are sequence specific transcription factors that are intimately involved in the regulation of the cell cycle. In addition to their role in cell cycle control, both E2F-1... -
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