Mycobiology.
2009 Sep;37(3):240-242. 10.4489/MYCO.2009.37.3.240.
An Efficient Method to Prepare PCR Cloning Vectors
- Affiliations
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- 1Polar BioCenter, Korea Polar Research Institute, KORDI, Songdo Techno Park, Songdo-dong 7-50, Yeonsu-gu, Incheon 406-840, Korea. polypore@kopri.re.kr
- 2Division of Plant Pathology and Microbiology, Department of Plant Sciences, College of Agriculture and Life Sciences, University of Arizona, Tucson, Arizona 85721, USA.
- KMID: 2053021
- DOI: http://doi.org/10.4489/MYCO.2009.37.3.240
Abstract
- An improved procedure for preparing PCR cloning vectors was developed. This procedure includes the incorporation of adapters to create XcmI restriction enzyme sites in pBluescript II SK(+) vectors, digestion with XcmI followed by further digestion of the small fragment produced by XcmI digestion with additional enzymes, and purification with PCR purification kits. Using this procedure, PCR cloning vectors with high ligation efficiencies and low blue or false-positive colonies were obtained.
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