- Comparison between Real-Time PCR and Agarose Gel Electrophoresis for DNA Quantification
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Lee MK, Kim HR
- KMID: 2238890
- Korean J Lab Med.
- 2006 Jun;26(3):217-222.
- doi: 10.3343/kjlm.2006.26.3.217
BACKGROUND: Real-time polymerase chain reaction (PCR) is generally regarded as a very accurate and time-saving method, but it is expensive to run. We evaluated the reliability of an inexpensive and... -
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- Detection of Methicillin Resistance in Staphylococcus aureus by 2% NaCl Mueller-Hinton Agar and Mannitol Salt Agar
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Lee CK, Ma KR, Lee DH, Whang SC, Kim YK, Lee KN
- KMID: 2082912
- Korean J Infect Dis.
- 1998 Dec;30(6):539-544.
BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) has a heteroresistant nature, so methicillin resistance is influenced by various culture conditions, such as temperature, incubation time, and NaCl content in the medium. Mueller... -
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- Oligoclonal Bands in Cerebrospinal fluid of Neurologic patients
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Kwon OS, Myong HJ
- KMID: 2044087
- J Korean Neurol Assoc.
- 1984 Dec;2(2):149-154.
The authors studied the diagnostic value of the oligoclonal band in cerebrospinal fluid in various neurological disorders. Cerebrospinal fluid of thirty-one patients including eight cases of multiple sclerosis was tested... -
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- Type III Hyperlipoproteinemia
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Lee BJ, Hann SK, Ahn SK, Im SB, Lee SH
- KMID: 2038875
- Korean J Dermatol.
- 1993 Apr;31(2):236-240.
We report a case of type III hyperlipoproteinemia which is called a broad-beta disease. A 53 year old female patient visited our clinic for the evaluation of multiple yellowish papules... -
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- Current status and perspectives of researches on programmed cell death in immune system
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Chung YH
- KMID: 2365358
- Allergy.
- 1995 Dec;15(4):569-595.
Programmed cell death(PCD), or apoptosis, is a distinct process of cell death in response to specific physiological or toxic signal. This genetically programmed form of cellular suicide is intimately involved... -
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- A Case of Multiple Myeloma Showing Marked Differences in Serum IgG Levels between Protein Electrophoresis and Turbidimetry
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Shin JW, Park R, Choi TY
- KMID: 854890
- Korean J Lab Med.
- 2008 Aug;28(4):282-285.
- doi: 10.3343/kjlm.2008.28.4.282
We report a case of multiple myeloma showing marked differences in serum Immunoglobulin G (IgG) levels between serum protein electrophoresis and turbidimetry. A 47-yr old man was admitted to our... -
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- Purification and characterization of lactate dehydrogenase from Varanus liver
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Javed MH M, Azimuddin , Hussain AN, Ahmed A, Ishaq M
- KMID: 2128622
- Exp Mol Med.
- 1997 Mar;29(1):25-30.
Lactate dehydrogenase was purified 21-fold from liver of Varanus bengalensis using colchicine-sepharose column chromatography. The crude enzyme showed two isoenzymes (LDH-5 and LDH-4) by agarose gel electrophoresis (AGE). The purified... -
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- Identification of Candida Species by Multiplex Polymerase Chain Reaction
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Lee MK, Kim HR, Lee YJ
- KMID: 2224724
- Korean J Clin Microbiol.
- 2006 Oct;9(2):119-124.
BACKGROUND: Polymerase chain reacation (PCR)-based methods have been described for rapid detection and identification of Candida spp. Multiplex PCR assay was developed using internal transcribed spacers and topoisomerase II gene... -
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- A Study on Prevalence of Premutation Sized FMR1 Gene Using Polymerase Chain Reaction
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Park W, Lee KJ, Choi EY
- KMID: 2007245
- J Korean Child Neurol Soc.
- 1999 Oct;7(1):42-47.
BACKGROUND: Fragile X syndrome is one of the most common causes of mental retardation. For its prevention, detection of premutation range CGG repeat in FMR1 gene is necessary. The aim... -
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- Gene Analysis of Probable Huntington's Disease
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Kim MH, Lee KW, Park KS, Jeon BS, Lee SB, Myung HJ
- KMID: 2342407
- J Korean Neurol Assoc.
- 1994 Jun;12(2):317-322.
Increased CAG repeats with full gene sequence (IT15) in Huntington's disease (HD) became known in 1993. We used polymerase chain reaction (PCR) in patients with clinically diagnosed Huntington's disease. After... -
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- Cholestasis Causes Discrepancy in HDL-Cholesterol Levels Measured Using Various Methods
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Kim S, Chun S, Lee W, Kim GS, Min WK
- KMID: 2312202
- Lab Med Online.
- 2012 Jul;2(3):174-178.
Herein, we report a case in which cholestasis caused discrepancy in high-density lipoprotein (HDL)-cholesterol levels measured using various methods. The discrepancy in HDL-cholesterol level originated from the abnormal increase in... -
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- Acetaminophen-Induced Hepatotoxicity in Cultured NCTC-1469 Cell Line
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Lee KE
- KMID: 2170968
- Ewha Med J.
- 1998 Dec;21(4):285-290.
- doi: 10.12771/emj.1998.21.4.285
Acetaminophen is a mild analgesic and antipyretic agent that is safe and effective when taken in therapeutic doses. Ingestion of overdoses, however, may lead to acute liver failure accompanied by... -
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- Identification and partial purification of pollen allergens from Artemisia princeps
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Park HS, Hong CS, Choi HJ, Hahm KS
- KMID: 1105563
- Yonsei Med J.
- 1989 Dec;30(4):346-354.
- doi: 10.3349/ymj.1989.30.4.346
The pollen of Artemisia has been considered as the main late summer-autumn allergen source in this country. To identify its allergenic components, Artemisia princeps pollen extracts were separated by... -
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- The Effect of Nitric Oxide on Cultured Bovine Retinal Pigment Epithelial Cell
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Lee DH, Yang HN
- KMID: 2336585
- J Korean Ophthalmol Soc.
- 1996 Dec;37(12):2048-2058.
Nitric oxide (NO), a potencially toxic radical, is generally regarded as a multi-potent molecule to be implicated in a wide range of biological function. The presence of nitric oxide synthase(NOS)... -
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- Serum Lipid Analysis Using Biphasic Electrophoresis
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Park JY, Choi HW, Lee JS, Cho D, Shin MG, Shin JH, Suh SP, Ryang DW
- KMID: 2200647
- J Lab Med Qual Assur.
- 2008 Jun;30(1):201-207.
BACKGROUND: Recently biphasic agarose gel electrophoresis method using Chol/Trig ComboTM for simultaneous detection of cholesterol and triglyceride on lipoprotein fractions has been developed to facilitate the classification and interpretation of... -
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- Toxicity of Bile Acids on Colon Cancer Cell Lines
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Park DK, Shin JH, Jun HJ, Kim KJ, Lee CY
- KMID: 2175280
- J Korean Cancer Assoc.
- 1999 Feb;31(1):126-133.
PURPOSE: Cytotoxicity of the bile acids on colon cancer cell lines was studied to know which bile acid was most cytotoxic to colonic mucosal epithelium. We performed agarose gel electrophoresis... -
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- Detection of Herpes Simplex Virus DNA in Clinical specimens by Polymerasde Chain Reaction (PCR)
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Jeon SJ, Kim KS, Baek WK, Seo SI, Seo MH
- KMID: 2336579
- J Korean Ophthalmol Soc.
- 1996 Dec;37(12):1996-2002.
The rapid and sensitive diagnostic methods for herpes simplex virus (HSV) infection have been developed. In this study, we employed the polymerase chain reaction (PCR) technique with primer 5 CATCACCGACCCGGAGACGGAC... -
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- Gene Analysis in Huntington Disease
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Jeon BS, Choi SH, Kim MH, Joo SI, Park SS
- KMID: 2342520
- J Korean Neurol Assoc.
- 1996 Jun;14(2):494-501.
BACKGROUND & OBJECTIVE: Huntington disease(HD) is clinically diagnosed by the triad of autosomal dominant inheritance, involuntary movements mainly chorea and dementia. The phenotype of HD is variable and other diseases... -
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- NRAMP1 Gene Expression in Patients with Kawasaki Disease
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Kim HY, Kim DS, Shin JS
- KMID: 2314218
- Pediatr Allergy Respir Dis.
- 2000 Jun;10(2):153-160.
PURPOSE: Kawasaki disease (KD) is considered to be an immunologic disease, although the exact etiology of the disease is still unknown. It is well known that the erythema on the... -
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- Rapid Apolipoprotein E Genotyping by the Multiplex Amplification Refractory Mutation System
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Lee MK, Park AJ
- KMID: 2089672
- Korean J Clin Pathol.
- 2001 Apr;21(2):154-159.
BACKGROUND: Currently, several different apolipoprotein E (apo E) genotyping methods have been developed. The Amplification Refractory Mutation System (ARMS) apo E genotyping, as previously described, requires four separate PCR reactions.... -
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