Yonsei Med J.  1989 Dec;30(4):346-354. 10.3349/ymj.1989.30.4.346.

Identification and partial purification of pollen allergens from Artemisia princeps

• Park HS
• Hong CS
• Choi HJ
• Hahm KS

• KMID: 1105563
• DOI: http://doi.org/10.3349/ymj.1989.30.4.346

Abstract

The pollen of Artemisia has been considered as the main late summer-autumn allergen source in this country. To identify its allergenic components, Artemisia princeps pollen extracts were separated by 10% sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE), and transferred to nitrocellulose membrane, where IgE binding components were detected by the reaction with sera of twenty Artemisia-allergic patients and 125I-anti-human IgE, sixteen components in the molecular range of 10,000 and 85,000 daltons were detected. Twelve bands bound to IgE from 50% of the sera tested, and two bands (37,000, 23,000 daltons) showed the highest (85%) frequency of IgE-binding in twenty sera tested. When the gel of SDS-PAGE with Artemisia pollen extracts was sliced into 11 allergenic groups (AG) and the protein of each AG was obtained by the gel elution method, the wormwool-RAST inhibition test showed that the AG 10 demonstrated to be the most potent, and the AG 7 was the next. Six AGs showed significant responses (more than 100% of wheal size to histamine, 1 mg/ml) on the skin prick test in more than 50% of the patients tested. It is suggested that electrophoretic transfer analysis with SDS-PAGE may be a valuable method for Artemisia allergen identification, and the possibility of partial purification of allergens by employing gel elution is discussed.

Keyword

Allergens; Artemisia princeps; electroblotting; gel elution

MeSH Terms

Blotting, Western/methods
Electrophoresis, Agar Gel/methods
Human
Korea
Lymphokines
Plants/immunology
Pollen/analysis/*immunology
Skin Tests/methods