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Luminex-based Immunoassay for Organ Transplantation

Lee H, Oh EJ

Development of luminex-based solid phase assays enables advanced measurement of HLA antibody with sensitivity, specificity, and increasing knowledge of unacceptable antigens. In this review, we described the principle of the...
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Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays

Choi IS, Yoo HS

  • KMID: 1095123
  • J Vet Sci.
  • 2002 Sep;3(3):207-212.
Interleukin-2 plays an important role in T lymphocyte proliferation and immune response regulations. In this study, porcine IL-2 cDNA was cloned from peripheral blood mononuclear cells, and recombinant porcine IL-2...
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Difference of Rh Phenotype between Irregular Antibody Positive Patients and RhD Positive Population in Korea

Kim KH, Kim BR, Choi JL, Woo KS, Kim JM, Han JY

  • KMID: 1705197
  • Korean J Blood Transfus.
  • 2014 Apr;25(1):60-68.
BACKGROUND: The Rh system is the most important blood group after ABO in the transfusion field. Nearly half of irregular antibodies with specificity are related to Rh antigens in Korea....
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A Case of Autoimmune Hemolytic Anemia Induced by Anti-e

Cho BW, Yoo SG, Kim JJ, Nahm CH, Kim CS, Choi JW, Pai SH

  • KMID: 1838440
  • J Korean Soc Microbiol.
  • 1999 Feb;34(1):61-68.
Autoimmune hemolytic anemia(AIHA) is characterized by the production of Coombs antibodies, which are responsible for the destruction of RBCs. The antibody specificity of warm A1HA(WAIHA) is very complex while all...
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A Case of Autoimmune Hemolytic Anemia Induced by Anti-e

Cho BW, Yoo SG, Nahm CH, Pai SH, Kim JJ, Kim CS

  • KMID: 1862943
  • Korean J Blood Transfus.
  • 1999 May;10(1):61-67.
Autoimmune hemolytic anemia (AIHA) is characterized by the production of Coombs' antibodies, which are responsible for the destruction of RBCs. The antibody specificity of warm AIHA (WAIHA) is very complex...
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Experience on Identification of Cross-Reactive Group Specificity Performed by Anti-human Globulin Panel Reactive Antibody Tests

Sohn YH, Cha CH, Kim MH, Ko SY, Oh HB

BACKGROUND: Panel reactive antibody (PRA) is to screen and identify HLA antibody. Majority of antibody specificities in high-PRA are directed against cross reactive group (CREG). Thus, this study was to...
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The Clinical Significance of Prenatal Antibody Screening Test

Chung HR, Park YM

  • KMID: 1863005
  • Korean J Blood Transfus.
  • 2005 Jun;16(1):14-19.
BACKGROUND: It is recommended that ABO, Rh typing and unexpected antibody screening should be tested during pregnancy in order to prevent hemolytic disease of the newborn (HDN). However, it is...
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Evaluation of In-house Lymphocyte Panel of 72 Wells for the Identification of HLA Antibody Specificity

Lee SG, Yang YS

  • KMID: 2089642
  • Korean J Clin Pathol.
  • 2000 Aug;20(4):419-423.
BACKGROUND: To detect anti-human leukocyte antigen(HLA) class I alloantibodies in patients awaiting solid organ transplantation, panel reactive antibody(PRA) test using complement-dependent lymphocytotoxicity(CDC) has been used. The enough size of lymphocyte...
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A Case of Anti-Sda Proven by Urine Neutralization Test

Yun SG, Kim JS, Yang JH, Yoon SY, Lee CK, Lim CS, Cho Y, Kim YK, Lee KN

  • KMID: 2312177
  • Lab Med Online.
  • 2011 Jan;1(1):64-66.
Anti-Sda is of no clinical significance, because it rarely causes hemolytic transfusion reactions. Even when its presence is suspected during antibody screening test, further identification of the antibody is usually...
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Development of a Web-based Program for the Identification of Human Leukocyte Antigen Antibody Specificities

Cha CH, Oh HB, Kim MH, Chae JM, Jung SY

BACKGROUND: Panel reactive antibody (PRA) test is used to determine whether a patient awaiting transplantation is previously sensitized. Tail analysis algorithm is widely used to identify antibody specificities, but...
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Development of decision support system for antibody identification

Choi KH, Han KS, Han BY, Suh JT, Kim SG, Cho HI

  • KMID: 2069256
  • Korean J Blood Transfus.
  • 1998 Dec;9(2):167-173.
BACKGROUND: Determination of antibody specificity using antigram spread sheet requires experience and knowledge on in vitro characteristics of red cell antibodies, time-consuming, and still subjective to human error. A computer-based...
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A monoclonal antibody to cell surface antigen of human thymic epithelial cell

Chung DH, Bae YM, Shin HY, Ahn HS, Song HG, Park WS, Park SH, Lee SK

The cell surface molecule identified by a monoclonal antibody(TE-1) to human thymic epithelial cell showed the specificity for thymic epithelial cells of both the cortex and medulla. TE-1 reacted with...
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Procurement of HLA Class I Antisera from Multiparous Blood Donors

Oh HB, Kwon SY, Kim SI

  • KMID: 2300544
  • Korean J Clin Pathol.
  • 1998 Dec;18(4):630-633.
BACKGROUND: HLA antisera are procured mainly from placental blood or blood of multiparous women. The latter has a merit that a large volume of antisera could be obtained, once the...
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Alloantibodies to High-Incidence Antigen: Review of Cases and Transfusion Experiences in Korea

Choi SJ, Chung YN, Cho D, Kim S

Antibodies to high-incidence red blood cell antigens should be considered if panagglutination reactions are noted in all panel cells, and negative reactions to autologous red blood cells are detected on...
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Comparison of NIH-CDC and AHG-CDC methods for the Detection of Panel Reactive Antibodies

Han TH, Kim SM, Lee YJ, Park MH

  • KMID: 2031036
  • Korean J Clin Pathol.
  • 1999 Oct;19(5):554-561.
BACKGROUND: Panel reactive antibody (PRA) test is used for anti-HLA antibody screening and characterization in patients awaiting organ transplantation. Complement-dependent cytotoxicity (CDC) is the most widely used standard procedure and...
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Irregular Antibody Positivity in Women Blood Donors with History of Parturition

Oh HB, Cho YJ, Kim SI, Kim DS, Han KS

  • KMID: 2253876
  • Korean J Blood Transfus.
  • 1997 May;8(1):39-47.
BACKGROUND: Antibody screening for donated blood is not yet being performed in Korea. Positive rate of irregular antibodies in Korean patients or blood donors has been thought to be much...
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Neonatal Alloimmune Thrombocytopenia Caused by Anti-HLA-A2 Alloantibodies Determined by Luminex Single Antigen Bead Assay

Kim Y, Oh EJ, Kim Y, Lee J, Sung IK

Neonatal alloimmune thrombocytopenia (NAIT) occurs when maternal alloantibodies react to antigens expressed on fetal platelets, which is mainly platelet-specific alloantigen or HLA, resulting in their immune destruction. Here, we described...
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Analysis of Discrepant Results between Enzyme Immunoassay and Anti-human Globulin -Complement-ependent Cytotoxicity Method for Panel Reactive Antibody Test

Hwang SH, Jang S, Oh HB, Im YH, Won CY

  • KMID: 2225969
  • Korean J Clin Pathol.
  • 2000 Oct;20(5):504-509.
BACKGROUND: Panel reactive antibody (PRA) test is important in that it could minimize the errorneous report of HLA crossmatch in emergent situation and be used as a information on nationwide...
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Positive Rate of HLA Class I Antibodies in Multiparous Korean Women

Song EY, Kim SM, Kim BC, Han KS, Park MH

  • KMID: 2225940
  • Korean J Clin Pathol.
  • 2000 Apr;20(2):210-214.
BACKGROUND: Anti-HLA antibodies are most frequently induced by transfusion or pregnancy, and these anibodies can be used as antisera for HLA typing. However these antibodies may elicit adverse reactions such...
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Comparative antibody response of five recombinant antigens in related to bacterial shedding levels and development of serological diagnosis based on 35 kDa antigen for Mycobacterium avium subsp. paratuberculosis

Shin SJ, Yoo HS, McDonough , Chang YF

  • KMID: 1120066
  • J Vet Sci.
  • 2004 Jun;5(2):111-117.
Eighty-five complex (85A, 85B and 85C), 35-kDa and superoxide dismutase (SOD) were cloned, expressed and purified as antigens in an enzyme-linked immunosorbent assay (ELISA) to compare the serological reactivity of...
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