Lab Med Online.  2011 Jan;1(1):64-66.

A Case of Anti-Sda Proven by Urine Neutralization Test

• Yun SG
• Kim JS
• Yang JH
• Yoon SY
• Lee CK
• Lim CS
• Cho Y
• Kim YK
• Lee KN

Abstract

Anti-Sda is of no clinical significance, because it rarely causes hemolytic transfusion reactions. Even when its presence is suspected during antibody screening test, further identification of the antibody is usually not performed. We experienced a case of anti-Sda in 73 yr-old male patient showing mixed field agglutination by microcolumn agglutination. Antibody specificity could not be identified by conventional antibody identification test, and it was proven to be anti-Sda by urine neutralization test. In spite of its little clinical significance, it may give incompatible crossmatching results reacting with Sda antigen, which occurs at a high frequency in general population. When incompatible crossmatch results arising from anti-Sda are suspected, the problem may be solved by using the urine-neutralized serum of in crossmatching test.

Keyword

Anti-Sda; Urine neutralization test; Mixed field agglutination

MeSH Terms

Agglutination
Antibody Specificity
Blood Group Incompatibility
Humans
Male
Mass Screening
Neutralization Tests

Figure

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  Fig. 1 Dialysis of pooled urine collected from 8 blood group O, Rh-positive individuals. After boiling, urine was dialyzed against PBS using dialysis tubing cellulose membrane (Sigma Aldrich).

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  Fig. 2 The result of anti-Sda neutralization test using urine. 1) Thawed urine + patient serum + 2% anti-Sda reactive screening cells, 2) PBS + patient serum + 2% anti-Sda reactive screening cells, 3) Thawed urine + PBS + 2% anti-Sda reactive screening cells.

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