Korean J Lab Med.  2008 Jun;28(3):221-229. 10.3343/kjlm.2008.28.3.221.

Effect of Interrupted Agitation and Removal of Leukocyte on Platelet Quality during the Storage of Platelet Concentrates

• Roh SG
• Joung US
• Choi WC
• Wee JH

• KMID: 854871
• DOI: http://doi.org/10.3343/kjlm.2008.28.3.221

Abstract

BACKGROUND
This study aimed to analyze the influence of the interruption of agitation and removal of leukocytes on platelet concentrates (PCs), and determine the maximum amount of time the agitation could be interrupted without impairing PCs' effectiveness during the storage period.
METHODS
Four ABO-identical random donor platelets agitated for 24 hr were pooled, and divided into 4 units, and 2 units of them were leukoreduced. Then 52 pooled units were categorized into 4 groups, non-leukoreduced continuous agitation (Non-LRCA), non-leukoreduced interrupted agitation (Non-LRIA), leukoreduced continuous agitation (LRCA), and leukoreduced interrupted agitation (LRIA), and preserved for 6 days (total 7 days). Mean platelet volume (MPV), pH, HCO3-, pO2, pCO2, CD62P, CD61, glucose, lactate, ammonia and free fatty acid were measured during the period.
RESULTS
Starting from the Day 4, the pH and HCO3- of Non-LRIA group begun to decrease while the amount of lactate production, glucose consumption, and MPV increased compared to the Non- LRCA group (P<0.01). An increase in pO2 level was observed in the interrupted agitation groups as the storage period prolonged (P<0.01). The pH levels of all the units in the agitation groups remained higher than 6.4 up to Day 7, while those of the non-leukoreduction group did so only up to Day 2, but those of leukoreduction in the interrupted agitation groups did so up to Day 4.
CONCLUSIONS
The interruption of agitation reduced the platelet's capacity to utilize oxygen, increasing lactate amount and reducing pH level. However, the in vitro parameters of the Non-LRIA and Non-LRCA groups on Day 2 were similar to each other and the pH level remained at 6.4 or higher, making one day of agitation interruption possible after 24 hr of agitation. With leukocytes removed, the effective agitation interruption period may become longer.

Keyword

Platelet concentrates; Interruption of agitation; Polyvinylchloride container; Platelet quality

MeSH Terms

*Blood Component Removal
Blood Platelets/*cytology
Blood Preservation/*standards
Cell Separation
Glucose/analysis
Humans
Hydrogen-Ion Concentration
Lactic Acid/blood
Oximetry
P-Selectin/blood
Time Factors
Vibration

Figure

• 

  Fig. 1. Metabolic measurements of platelets concentrations with a Non-LRCA (□), Non-LRIA (Δ), LRCA (▪) and LRIA (□) during the storage period. The results show mean standard deviation (SD) and error bars (n=13). (Glucose and Lactate: Day 4, 7 P<0.01) P values were calculated by Mann-Whitney test and compared between groups in the same storage period.

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