Korean J Intern Med.  2003 Mar;18(1):1-5.

Airway Obstruction after Acute Ozone Exposure in BALB/c Mice Using Barometric Plethysmography

• Jang AS
• Choi IS
• Kim SW
• Song BC
• Yeum CH
• Jung JY

Abstract

BACKGROUND
Airway responsiveness after acute inhalation of ozone is related to the concentration and duration of ozone exposure. Using barometric whole-body plethysmography and increase in enhanced pause (Penh) as an index of airway obstruction, we measured the response of BALB/c mice to acute ozone inhalation to study the time course change of pulmonary function after ozone exposure. METHODS: Penh was measured before and after exposure to filtered air or 0.12, 0.5, 1, or 2 ppm ozone for 3 hr (n=6/group). In addition, Penh was measured 24, 48 and 72 hr after ozone exposure. Bronchoalveolar lavage (BAL) and histopathologic examinations were performed. RESULTS: The increase in Penh after ozone exposure was significantly higher in the 0.12, 0.5, 1 and 2 ppm groups compared with the control group (all p< 0.01). Increases in Penh 24 hr after ozone exposure were significantly lower than those immediately after acute ozone exposure; however, increases in Penh 72 hr after ozone exposure were significantly higher than those in the control group (each p< 0.01). The proportion of neutrophils in BAL fluid was significantly higher in the group exposed to 2 ppm ozone than in the groups exposed to filtered air or 0.12 ppm ozone (both p< 0.01). CONCLUSION: These results indicate that airway obstruction is induced following ozone exposure in a concentration-dependent manner and persists for at least 72 hr.

Keyword

Ozone; Airway obstruction; Time

MeSH Terms

Airway Obstruction/*etiology/*pathology
Animals
Animals, Newborn
Bronchoalveolar Lavage Fluid/cytology
Disease Models, Animal
Female
Mice
Mice, Inbred BALB C
Plethysmography, Whole Body/*methods
Probability
Reference Values
Respiratory Function Tests
Risk Assessment
Sensitivity and Specificity
Statistics, Nonparametric
Sulfuric Acids/*adverse effects