Exp Mol Med.
2004 Feb;36(1):52-56.
Forskolin promotes astroglial differentiation of human central neurocytoma cells
- Affiliations
-
- 1Department of Anatomy, School of Medicine, Ajou University, Suwon 442-749, Korea. hysuh@ajou.ac.kr
- 2Department of Neurosurgery, Seoul National University College of Medicine, Seoul 110-749, Korea.
- 3Brain Disease Research Center, School of Medicine, Ajou University, Suwon 442-749, Korea.
Abstract
- Human central neurocytoma is a kind of the brain tumors that are usually found in anterior part of the lateral ventricles. In this study, we established conditions that allowed proliferation of neurocytoma cells culture and analyzed characteristics of neurocytoma cells in vitro. For in vitro, a condition that used for culturing neural stem cells and contained basic fibroblast growth factor (bFGF) provided high proliferation. RT-PCR analaysis showed that nestin was found in neurocytoma cells, indicating that the neurocytomas possess neural stem cell properties. Interestingly, treatment of neurocytoma cells with forskolin increased expression of glial fibrillary acidic protein with a concomitant decrease in the nestin expression. Forskolin also induced morphological changes of neurocytoma cells to adopt an astrocyte-like phenotype. The results suggest that neurocyotma cells may have properties of multipotent neural stem cells.
MeSH Terms
-
Animals
Astrocytes/cytology/*physiology
Cell Differentiation/*drug effects
Cell Proliferation
Cell Shape
Fibroblast Growth Factor 2/pharmacology
Forskolin/*pharmacology/*therapeutic use
Humans
Intermediate Filament Proteins/metabolism
Nerve Tissue Proteins/metabolism
Neurocytoma/*drug therapy/pathology
Research Support, Non-U.S. Gov't
Tumor Cells, Cultured
