Korean J Radiol.  2002 Sep;3(3):194-198. 10.3348/kjr.2002.3.3.194.

Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration

• Choi BY
• Chung JW
• Park JH
• Kim KH
• Kim YI
• Koh YH
• Kwon JW
• Lee KH
• Choi HJ
• Kim TW
• Kim YJ
• Chung H
• Kwon IC
• Jeong SY

• KMID: 754062
• DOI: http://doi.org/10.3348/kjr.2002.3.3.194

Abstract

OBJECTIVE
To compare the efficiency of intra-arterial, intraportal, and intravenous administration of cationic lipid emulsion/DNA complex, as used for gene transfer to rat liver.
MATERIALS AND METHODS
DNA-carrier complex for the in-vivo experiment was prepared by mixing DNA and a cationic lipid emulsion. According to the administration route used (intra-arterial, intraportal, or intravenous), the animals were assigned to one of three groups. The heart, lung, liver, spleen and kidneys were removed and assayed for total protein and luciferase concentration.
RESULTS
The cationic lipid emulsion/DNA complex used successfully transfected the various organs via the different administration routes employed. Luciferase activity in each organ of untreated animals was negligible. Liver luciferase values were significantly higher in the groups in which intra-arterial or intraportal administration was used.
CONCLUSION
The intra-arterial or intraportal administration of cationic lipid emulsion/DNA complex is superior to intravenous administration and allows selective gene transfer to the liver.

Keyword

Animals; Genes and genetics; Liver, interventional procedure

MeSH Terms

Animal
Catheterization
Cations/*administration & dosage
Comparative Study
DNA/*administration & dosage/genetics
Emulsions/administration & dosage
Gene Transfer Techniques
Injections, Intra-Arterial
Injections, Intravenous
Lipids/*administration & dosage/genetics
Liver/*metabolism
Rats
Rats, Sprague-Dawley

Figure

• 

  Fig. 1 The graph illustrates average and standard deviation of the amount of luciferase enzyme in each organ of the three groups which underwent, respectively, intra-arterial (IA), intraportal (IP), and intravenous (IV) administration.

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