Korean J Parasitol.
2004 Sep;42(3):129-135. 10.3347/kjp.2004.42.3.129.
Sequence comparisons of 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I of Metagonimus yokogawai, M. takahashii and M. miyatai
- Affiliations
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- 1Department of Parasitology, College of Medicine, Hallym University, Chuncheon 200-702, Repubic of Korea. shuh@hallym.ac.kr
- 2Department of Parasitology, Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju 660-751, Repubic of Korea.
- 3Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Repubic of Korea.
- KMID: 662783
- DOI: http://doi.org/10.3347/kjp.2004.42.3.129
Abstract
- We compared the DNA sequences of the genus Metagonimus: M. yokogawai, M. takahashii, and M. miyatai. We obtained 28S D1 ribosomal DNA (rDNA) and mitochondrial cytochrome c oxidase subunit I (mtCOI) fragments from the adult worms by PCR, that were cloned and sequenced. Phylogenetic relationships inferred from the nucleotide sequences of the 28S D1 rDNA and mtCOI gene. M. takahashii and M. yokogawai are placed in the same clade supported by DNA sequence and phylogenic tree analysis in 28S D1 rDNA and mtCOI gene region. The above findings tell us that M. takahashii is closer to M. yokogawai than to M. miyatai genetically. This phylogenetic data also support the nomination of M. miyatai as a separate species.
Keyword
MeSH Terms
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Animals
Base Sequence
Comparative Study
DNA, Helminth/*chemistry/genetics
DNA, Mitochondrial/chemistry/genetics
DNA, Ribosomal/chemistry/genetics
Electron Transport Complex IV/chemistry/*genetics
Heterophyidae/classification/enzymology/*genetics
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 28S/chemistry/*genetics
Research Support, Non-U.S. Gov't
Sequence Alignment
Trematode Infections/*parasitology
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