Korean J Parasitol.  2007 Sep;45(3):181-189. 10.3347/kjp.2007.45.3.181.

Molecular phylogeny of parasitic Platyhelminthes based on sequences of partial 28S rDNA D1 and mitochondrial cytochrome c oxidase subunit I

• Lee SU
• Chun HC
• Huh S

• KMID: 1104528
• DOI: http://doi.org/10.3347/kjp.2007.45.3.181

Abstract

The phylogenic relationships existing among 14 parasitic Platyhelminthes in the Republic of Korea were investigated via the use of the partial 28S ribosomal DNA (rDNA) D1 region and the partial mitochondrial cytochrome c oxidase subunit 1 (mCOI) DNA sequences. The nucleotide sequences were analyzed by length, G + C %, nucleotide differences and gaps in order to determine the analyzed phylogenic relationships. The phylogenic patterns of the 28S rDNA D1 and mCOI regions were closely related within the same class and order as analyzed by the PAUP 4.0 program, with the exception of a few species. These findings indicate that the 28S rDNA gene sequence is more highly conserved than are the mCOI gene sequences. The 28S rDNA gene may prove useful in studies of the systematics and population genetic structures of parasitic Platyhelminthes.

Keyword

Platyhelminthes; molecular phylogeny; 28S ribosomal DNA; mitochondrial cytochrome c oxidase subunit 1

MeSH Terms

Animals
Base Sequence
Cats
Cestoda/*classification/genetics
Cestode Infections/parasitology
Conserved Sequence
DNA Primers/chemistry
DNA, Ribosomal/chemistry
Electron Transport Complex IV/*genetics
Humans
Korea
Mice
Mice, Inbred BALB C
Molecular Sequence Data
*Phylogeny
RNA, Ribosomal, 28S/*genetics
Rats
Rats, Sprague-Dawley
Sequence Analysis, DNA
Trematoda/*classification/genetics
Trematode Infections/parasitology